Time-gated spectroscopy of intrinsic fluorophores in cells and tissues

Herbert Schneckenburger; Michael H. Gschwend; Ruediger J. Paul; Herbert G. Stepp; Kai Rick; Volkmar Betz; Wolfgang S. L. Strauss

doi:10.1117/12.198719

4 January 1995 Time-gated spectroscopy of intrinsic fluorophores in cells and tissues

Herbert Schneckenburger, Michael H. Gschwend, Ruediger J. Paul, Herbert G. Stepp, Kai Rick, Volkmar Betz, Wolfgang S. L. Strauss

Author Affiliations +

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198719
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Based on novel time-resolving methods, the autofluorescence of saccharomyces, cultivated endothelial cells and biopsy specimens of human bladder was measured. The emission was found to be composed by the coenzymes NADH (free and protein-bound) and flavins with a concentration ratio of 100:1 between free NADH and flavin molecules. The fluorescence intensity of free NADH appeared to be a measure of the respiratory function. In addition, epithelial and connective tissues could be distinguished by the intensity ratio at 435 and 460 nm, which may be affected by the relation of bound and free NADH, but also by the extracellular fluorescence of elastin.

Citation Download Citation

Herbert Schneckenburger, Michael H. Gschwend, Ruediger J. Paul, Herbert G. Stepp, Kai Rick, Volkmar Betz, and Wolfgang S. L. Strauss "Time-gated spectroscopy of intrinsic fluorophores in cells and tissues", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198719

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