Photothermal imaging

Dmitry Lapotko; Elena Antonishina

doi:10.1117/12.200892

1 February 1995 Photothermal imaging

Dmitry Lapotko, Elena Antonishina

Proceedings Volume 2329, Optical and Imaging Techniques in Biomedicine; (1995) https://doi.org/10.1117/12.200892
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

An automated image analysis system with two imaging regimes is described. Photothermal (PT) effect is used for imaging of a temperature field or absorption structure of the sample (the cell) with high sensitivity and spatial resolution. In a cell study PT-technique enables imaging of live non-stained cells, and the monitoring of the cell shape/structure. The system includes a dual laser illumination unit coupled to a conventional optical microscope. A sample chamber provides automated or manual loading of up to 3 samples and cell positioning. For image detection a 256 X 256 10-bit CCD-camera is used. The lasers, scanning stage, and camera are controlled by PC. The system provides optical (transmitted light) image, probe laser optical image, and PT-image acquisition. Operation rate is 1 - 1.5 sec per cell for a cycle: cell positioning -- 3 images acquisition -- image parameters calculation. A special database provides image/parameters storage, presentation, and cell diagnostic according to quantitative image parameters. The described system has been tested during live and stained blood cell studies. PT-images of the cells have been used for cell differentiation. In experiments with the red blood cells (RBC) that originate from normal and anaemia blood parameters for disease differentiation have been found. For white blood cells in PT-images the details of cell structure have found that absent in their optical images.

Citation Download Citation

Dmitry Lapotko and Elena Antonishina "Photothermal imaging", Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); https://doi.org/10.1117/12.200892

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available