Spark source infrared fluorometry

Graham Hungerford; David J. S. Birch; Robert E. Imhof

doi:10.1117/12.58218

1 April 1992 Spark source infrared fluorometry

Graham Hungerford, David J. S. Birch, Robert E. Imhof

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58218
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

The application of our development of 750 nm excitation from an argon-hydrogen filled spark source to time-resolved fluorescence probe studies of lipid membranes and inverse micelles is reported. The laser dye IR-140 was studied using the single-photon counting technique both in a lipid membrane of L-(alpha) dipalmitoylphosphatidylcholine (DPPC) and in sodium sulfosuccinic acid bis (2-ethylhexyl) ester (AOT) in iso-octane. In DPPC a dramatic change in the fluorescence behavior of IR-140 is observed between the gel and liquid crystalline phases. In inverse micelles of AOT an increase in the intensity of the peak fluorescence emission and a decrease in fluorescence lifetime is noticed on increasing the water content.

Citation Download Citation

Graham Hungerford, David J. S. Birch, and Robert E. Imhof "Spark source infrared fluorometry", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58218

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