Long-wavelength derivatization reagents for use in diode laser-induced fluorescence detection

H. Thomas Karnes; Sadayappan V. Rahavendran; Min Gui

doi:10.1117/12.208489

8 May 1995 Long-wavelength derivatization reagents for use in diode laser-induced fluorescence detection

H. Thomas Karnes, Sadayappan V. Rahavendran, Min Gui

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208489
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

A diode laser fluorometer was constructed and evaluated using rhodamine 800, a commercially available laser dye. Rhodamine 800 was evaluated as a precolumn derivatization reagent through an intermediate for conjugation to amine containing analytes. Preliminary results show that the derivative is nonfluorescent. Rhodamine 800 was successfully used as a mobile phase additive for HPLC with indirect detection of valproic acid from plasma. Nile blue, was evaluated as a derivatization reagent for carboxyl containing analytes. A limit of detection of 0.96 pmol was observed for the nile blue conjugate of benzoic acid using a conventional fluorescence detector. A cyanine dye, was used as a near infrared labeling reagent for compounds containing primary amine functionality. The spectral properties of the cyanine derivative of 2-adamantanamine were similar to the unreacted label and very favorable for low level detection.

Citation Download Citation

H. Thomas Karnes, Sadayappan V. Rahavendran, and Min Gui "Long-wavelength derivatization reagents for use in diode laser-induced fluorescence detection", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208489

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available