Recombinant Adeno-associated virus (rAAV)-mediated transduction and optogenetic manipulation of cortical neurons in vitro

Wienke Lange; Lei Jin; Vanessa Maybeck; Annika Meisenberg; Arnd Baumann; Andreas Offenhäusser

doi:10.1117/12.2038437

24 March 2014 Recombinant Adeno-associated virus (rAAV)-mediated transduction and optogenetic manipulation of cortical neurons in vitro

Wienke Lange, Lei Jin, Vanessa Maybeck, Annika Meisenberg, Arnd Baumann, Andreas Offenhäusser

Author Affiliations +

Proceedings Volume 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 89282S (2014) https://doi.org/10.1117/12.2038437
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Genetically encoded light-sensitive proteins can be used to manipulate and observe cellular functions. According to different modes of action, these proteins are divided into actuators like the blue-light gated cation channel Channelrhodopsin-2 (ChR2) and detectors like the calcium sensor GCaMP. In order to optogenetically control and study the activity of rat primary cortical neurons, we established a transduction procedure using recombinant Adeno-associated viruses (rAAVs) as gene-ferries. Thereby, we achieved high transduction rates of these neurons with ChR2. In ChR2 expressing neurons, action potentials could be repeatedly and precisely elicited with laser pulses and measured via patch clamp recording.

Citation Download Citation

Wienke Lange, Lei Jin, Vanessa Maybeck, Annika Meisenberg, Arnd Baumann, and Andreas Offenhäusser "Recombinant Adeno-associated virus (rAAV)-mediated transduction and optogenetic manipulation of cortical neurons in vitro", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89282S (24 March 2014); https://doi.org/10.1117/12.2038437

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