Improvements in low-cost label-free QPI microscope for live cell imaging

C. Seniya; C. E. Towers; D. P. Towers

doi:10.1117/12.2282230

28 July 2017 Improvements in low-cost label-free QPI microscope for live cell imaging

C. Seniya, C. E. Towers, D. P. Towers

Proceedings Volume 10414, Advances in Microscopic Imaging; 104140F (2017) https://doi.org/10.1117/12.2282230
Event: European Conferences on Biomedical Optics, 2017, Munich, Germany

Abstract

This paper reports an improvement in the development of a low-cost QPI microscope offering new capabilities in term of phase measurement accuracy for label-free live samples in the longer term (i.e., hours to days). The spatially separated scattered and non-scattered image light fields are reshaped in the Fourier plane and modulated to form an interference image at a CCD camera. The apertures that enable these two beams to be generated have been optimised by means of laser-cut apertures placed on the mirrors of a Michelson interferometer and has improved the phase measuring and reconstruction capability of the QPI microscope. The microscope was tested with transparent onion cells as an object of interest.

Citation Download Citation

C. Seniya, C. E. Towers, and D. P. Towers "Improvements in low-cost label-free QPI microscope for live cell imaging", Proc. SPIE 10414, Advances in Microscopic Imaging, 104140F (28 July 2017); https://doi.org/10.1117/12.2282230

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