We evaluated the application of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for the assessment of histopathological inflammation in patients with Ulcerative colitis (UC). Endoscopically obtained colonic and rectal mucosal biopsy samples from 21 patients with UC were analyzed by capturing DHM QPI images from which subsequently the subepithelial refractive index (RI) was determined. The retrieved RI data were correlated with established histological scoring systems including the Nancy index (NI) as well as with endoscopic and clinical findings. In conclusion, our data demonstrate DHM as a promising tool for quantitative assessment of mucosal inflammation in patients with UC.
In overview, for the example of digital holographic microscopy (DHM), we present quantitative phase imaging (QPI)- based characterization of inflammation in colonic tissue sections. Tissue density alterations are determined by the refractive index retrieval from quantitative DHM phase images. We show that the average tissue refractive index represents a marker that allows to distinguish between different layers of the intestinal wall and that the revealed tissue refractive index data correlated well with the severity of inflammation. In summary, QPI provides biophysical parameters for the quantification of inflammation in intestinal tissues and paves the way for future applications in terms of ‘‘digital pathology’’.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.