KEYWORDS: Fluorescence lifetime imaging, 3D acquisition, 3D image processing, Microscopy, Image acquisition, Image processing, Current controlled current source, Luminescence, Physical phenomena, Testing and analysis
We compare various time-gated and discrete-time methods for extracting the decay constant from an exponential signal, typical of fluorescence lifetime (FLIM) instruments, as well as a host of other physical phenomena. Analytical methods are evaluated for accuracy and precision as well as for computational and sampling efficiency.
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