Dr. Elli Kohen Profile

Dr. Elli Kohen

at Univ of Miami

SPIE Involvement:

Author

Publications (8)

Proceedings Article | 14 October 2003 Paper

Toward Fourier interferometry fluorescence excitation/emission imaging of malignant cells combined with photoacoustic microscopy

Elli Kohen, Joseph Hirschberg, John Berry, Nuri Ozkutuk, Ceren Ornek, Marco Monti, Roger Leblanc, Dietrich Schachtschabel, Sumaira Haroon

Proceedings Volume 5143, (2003) https://doi.org/10.1117/12.500999

KEYWORDS: Luminescence, Glucose, Photoacoustic microscopy, Interferometry, Imaging spectroscopy, Colorectal cancer, Yeast, Mode conditioning cables, Biomedical optics, Oxygen

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Proceedings Article | 24 October 2001 Paper

Fourier interferometry with spectral fluorescence imaging for the study of mitochondrial distribution and function in living normal/malignant cells

Joseph Hirschberg, Elli Kohen, Cahide Kohen, Ceren Oernek, John Berry

Proceedings Volume 4434, (2001) https://doi.org/10.1117/12.446674

KEYWORDS: Luminescence, Interferometers, Hydrogen, Interferometry, Yeast, Biomedical optics, Biotechnology, Mirrors, Michelson interferometers, Solids

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Proceedings Article | 27 April 2000 Paper

Fluorescence spectral imaging of organelle interaction

Elli Kohen, Joseph Hirschberg, Cahide Kohen, Dietrich Schachtschabel, Marco Monti, Rita Stanikunaite

Proceedings Volume 3921, (2000) https://doi.org/10.1117/12.384215

KEYWORDS: Luminescence, Interferometers, Prisms, Mirrors, Imaging spectroscopy, Beam splitters, Yeast, Michelson interferometers, Melanoma, Microscopes

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Proceedings Article | 3 May 1999 Paper

Nad(P)H vs. Schiff base fluorescence by spectroscopy, imaging, and maximum sensitivity micrographs at the convergence of cellular detoxification, senescence, and transformation

Elli Kohen, Joseph Hirschberg, Cahide Kohen, Marco Monti

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347520

KEYWORDS: Luminescence, Imaging spectroscopy, Melanoma, Image quality, Charge-coupled devices, Mode conditioning cables, Spectroscopy, Fluorescence spectroscopy, Glucose, Photomicroscopy

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Proceedings Article | 1 February 1995 Paper

Toward noninvasive microspectrofluorometry of skin lesions for diagnostic and prognostic evaluation of cell metabolism and organelle interactions

Joseph Hirschberg, Astrid Schachtschabel, Elli Kohen, Cahide Kohen, Dietrich Schachtschabel

Proceedings Volume 2329, (1995) https://doi.org/10.1117/12.200886

KEYWORDS: Luminescence, Skin, Melanoma, Mode conditioning cables, Modulation, Diagnostics, Ultraviolet radiation, Head, Objectives, Fetus

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The basic principle of this approach relies on microspectrofluorometric observations of upheavals in the cell's energy metabolism and cell-to-cell metabolic communication in human and mouse melanoma cells. A striking feature is the definition of a highly active nuclear energy metabolism in M8255 human melanoma cells which is characterized by an intense fluorescence response associated with NAD(P) reduction by substrates of glycolysis or the hexose monophosphate shunt. Changes are also expected in the steady state levels of reduced/oxidized NAD(P) in the nuclear, cytoplasmic and mitochondrial compartments, which are probably dependent on ATP levels and distribution (as determined by cell metabolism and eventually the presence of ATP traps). A topographic scanning of skin lesions, either under metabolic steady state conditions or in the presence of permeating substrates, can lead to the recognition of characteristic patterns associated with pigmented and nonpigmented, malignant and nonmalignant skin lesions. The method is, in a way, an extension of microscopic transillumination techniques which have led to the identification of specific patterns associated with such lesions. However, here, a new dimension is added by introduction of fluorescence evaluations. This can represent the first step in a multiparameter approach to the non-invasive in situ fluorescence scan of dermatological lesions by inclusion of: (1) fluorescence excitation and emission spectra; (2) new fluorescence probes of cytoplasmic organelles and nuclear components. Primary emphasis should be placed on the highly active nuclear energy metabolism, which can be triggered to maximum levels when the role of mitochondria as the `cells's policeman' with regard to metabolic control is suppressed by use of topically cytotoxic agents such as the `antipsoriatic' anthralin and dicarboxylic acids used in the local treatment of melanoma. Fluorescence excitation spectroscopy may be of particular advantage in studies with the new highly sensitive cyanine nucleic acid dyes and their dimers, but caution should be exerted in the use of such compounds because of cytotoxicity (e.g., limiting it to cellular studies used in the interpretation of dermatological findings). Parallel cellular and non-invasive dermatological studies will help to define the most specific set of parameters to be used in diagnostic and prognostic evaluations of skin lesions.

Proceedings Article | 1 February 1994 Paper

Microspectrofluorometry for metabolic control analysis and the study of organelle morphogenesis in cell differentiation and transformation

Joseph Hirschberg, Elli Kohen, Cahide Kohen, Raul Pinon

Proceedings Volume 2083, (1994) https://doi.org/10.1117/12.167414

KEYWORDS: Luminescence, Melanoma, Mode conditioning cables, Glucose, Near field scanning optical microscopy, Bioenergetics, Acquisition tracking and pointing, Photomicroscopy, Yeast, Biological research

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