Dr. Iris Riemann Profile

Dr. Iris Riemann

at Fraunhofer-Institut für Biomedizinische Technik

SPIE Involvement:

Author

Publications (58)

Proceedings Article | 18 February 2011 Paper

Paper

Intravital multiphoton tomography as an appropriate tool for non-invasive in vivo analysis of human skin affected with atopic dermatitis

Volker Huck, Christian Gorzelanny, Kai Thomas, Christian Mess, Valentina Dimitrova, Martin Schwarz, Iris Riemann, Verena Niemeyer, Thomas Luger, Karsten König, Stefan Schneider

Proceedings Volume 7883, 78830R (2011) https://doi.org/10.1117/12.874218

KEYWORDS: Skin, In vivo imaging, Luminescence, Fluorescence lifetime imaging, Inflammation, Multiphoton tomography, Tomography, Mode conditioning cables, Pathogens, Biological research

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Proceedings Article | 2 March 2010 Paper

Paper

Evaluation of cryo-preserved skin tissues using two-photon microscopy

Iris Riemann, Axel Beier, Martin Schwarz, Daniel Dörr, Frank Stracke, Heiko Zimmermann

Proceedings Volume 7548, 754804 (2010) https://doi.org/10.1117/12.840862

KEYWORDS: Tissues, Skin, Biopsy, Collagen, Crystals, Two photon excitation microscopy, Second-harmonic generation, Multiphoton microscopy, Stem cells, Luminescence

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Proceedings Article | 24 February 2010 Paper

Paper

A comparative study of different instrumental concepts for spectrally and lifetime-resolved multiphoton intravital tomography (5D-IVT) in dermatological applications

M. Schwarz, I. Riemann, F. Stracke, V, Huck, C. Gorzelanny, S. Schneider, K. König, S. Puschmann, V. Lutz, N. Sommer, C. Rahn, S. Gallinat, H. Wenck, K.-P. Wittern, F. Fischer

Proceedings Volume 7568, 75680D (2010) https://doi.org/10.1117/12.840843

KEYWORDS: Luminescence, Collagen, Tomography, Spectrographs, Diffraction gratings, Single photon, Skin, Fluorescence lifetime imaging, Temporal resolution, CCD cameras

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Proceedings Article | 23 February 2010 Paper

Paper

5D-intravital tomography as a novel tool for non-invasive in-vivo analysis of human skin

Karsten König, Martin Weinigel, Hans Breunig, Axel Gregory, Peter Fischer, Marcel Kellner-Höfer, Rainer Bückle, Martin Schwarz, Iris Riemann, Frank Stracke, Volker Huck, Christian Gorzelanny, Stefan Schneider

Proceedings Volume 7555, 75551I (2010) https://doi.org/10.1117/12.841861

KEYWORDS: Skin, Tissue optics, Luminescence, Fluorescence lifetime imaging, In vivo imaging, Imaging systems, Tomography, Harmonic generation, 3D image processing, Single photon

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Proceedings Article | 3 March 2009 Paper

Paper

New developments in two photon endoscopy

M. Schwarz, I. Riemann, M. Weinigel, K. König, B. Messerschmidt, R. Le Harzic

Proceedings Volume 7172, 717204 (2009) https://doi.org/10.1117/12.808781

KEYWORDS: Zoom lenses, Endoscopes, Scanners, Luminescence, Objectives, Optical fibers, Endoscopy, GRIN lenses, Beam splitters, Fluorescence spectroscopy

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Proceedings Article | 13 February 2009 Paper

Paper

New developments in two-photon analysis of human skin in vivo

I. Riemann, M. Schwarz, F. Stracke, A. Ehlers, E. Dimitrow, M. Kaatz, K. König, R. Le Harzic

Proceedings Volume 7203, 720306 (2009) https://doi.org/10.1117/12.808770

KEYWORDS: Skin, Tissues, Fluorescence lifetime imaging, In vivo imaging, Collagen, Melanoma, Second-harmonic generation, Atrial fibrillation, Biopsy, Multiphoton tomography

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Proceedings Article | 21 February 2008 Paper

Paper

Two-photon imaging using a flexible endoscope

Iris Riemann, Selma Schenkl, Ronan Le Harzic, Daniel Sauer, Alexander Ehlers, Bernhard Messerschmidt, Rainer Bückle, Karsten König

Proceedings Volume 6851, 68510B (2008) https://doi.org/10.1117/12.762970

KEYWORDS: Luminescence, Endoscopes, Tissues, Photonic crystal fibers, Scanners, Fiber lasers, Two photon imaging, Microscopes, GRIN lenses, Image resolution

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Proceedings Article | 15 February 2008 Paper

Paper

Two-photon imaging of stem cells

A. Uchugonova, E. Gorjup, I. Riemann, D. Sauer, K. König

Proceedings Volume 6860, 68601W (2008) https://doi.org/10.1117/12.762734

KEYWORDS: Stem cells, Luminescence, Second-harmonic generation, Picosecond phenomena, Signal detection, Two photon imaging, Fluorescence lifetime imaging, Femtosecond phenomena, Imaging spectroscopy, Collagen

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Proceedings Article | 8 February 2008 Paper

Paper

Non-invasive analysis/diagnosis of human normal and melanoma skin tissues with two-photon FLIM in vivo

Iris Riemann, Alexander Ehlers, Ronan Le Harzic, Enrico Dimitrow, Martin Kaatz, P. Elsner, Rainer Bückle, Karsten König

Proceedings Volume 6842, 684205 (2008) https://doi.org/10.1117/12.762937

KEYWORDS: Tissues, Melanoma, Skin, Fluorescence lifetime imaging, In vivo imaging, Biopsy, Proteins, Biological research, Optical tomography, Luminescence

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Proceedings Article | 23 July 2007 Paper

Paper

In vivo multiphoton tomography: a non invasive powerful tool for biochemical investigation of human skin

Ronan Le Harzic, Anne Colonna, Rainer Bückle, Alexander Ehlers, Christophe Hadjur, Frederic Leroy, Frederic Flament, Roland Bazin, Bertrand Piot, Iris Riemann, Karsten König

Proceedings Volume 6630, 66300V (2007) https://doi.org/10.1117/12.727044

KEYWORDS: Atrial fibrillation, Second-harmonic generation, Collagen, Skin, In vivo imaging, Harmonic generation, Multiphoton tomography, Single photon, Photomultipliers, Biomedical optics

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Proceedings Article | 18 July 2007 Paper

Paper

Rigid and high NA multiphoton fluorescence GRIN-endoscopes

Selma Schenkl, Alexander Ehlers, Ronan Le Harzic, Martin Stark, Iris Riemann, Bernhard Messerschmidt, Martin Kaatz, Karsten König

Proceedings Volume 6631, 66310Q (2007) https://doi.org/10.1117/12.728503

KEYWORDS: Skin, Imaging systems, Luminescence, GRIN lenses, Image resolution, Collagen, In vivo imaging, Endoscopes, Signal detection, Microscopes

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Proceedings Article | 23 March 2007 Paper

Paper

Multiphoton tomography of skin tumors after ALA application

Iris Riemann, Alexander Ehlers, Dorothee Dill-Müller, Sven Martin, Karsten König

Proceedings Volume 6424, 642405 (2007) https://doi.org/10.1117/12.702411

KEYWORDS: Skin, Photodynamic therapy, Luminescence, Multiphoton tomography, Biopsy, Tissues, Tumors, Skin cancer, Near infrared, Therapeutics

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Proceedings Article | 15 February 2007 Paper

Paper

Applications of rigid and flexible GRIN-endoscopes

Selma Schenkl, Alexander Ehlers, Iris Riemann, Bernhard Messerschmidt, Rainer Bückle, Karsten König

Proceedings Volume 6433, 64330N (2007) https://doi.org/10.1117/12.702413

KEYWORDS: Endoscopes, Imaging systems, GRIN lenses, Objectives, Microscopes, Collagen, Luminescence, Skin, Tissues, Signal detection

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Proceedings Article | 13 February 2007 Paper

Paper

Imaging living cells with a combined high-resolution multi-photon-acoustic microscope

Selma Schenkl, Eike Weiss, Martin Stark, Frank Stracke, Iris Riemann, Robert Lemor, Karsten König

Proceedings Volume 6437, 64372A (2007) https://doi.org/10.1117/12.702391

KEYWORDS: Acoustics, Microscopes, Ultrasonography, Luminescence, Microscopy, Sapphire, Imaging systems, Absorption, Image resolution, In vivo imaging

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Proceedings Article | 12 February 2007 Paper

Paper

Novel concept of GRIN optical systems for high resolution microendoscopy: Part 1. Physical aspects

Bernhard Messerschmidt, Anke Kraeplin, Selma Schenkl, Iris Riemann, Martin Stark, Alexander Ehlers, Andrei Tchernook, Ronan Le Harzic, Karsten Koenig

Proceedings Volume 6432, 643202 (2007) https://doi.org/10.1117/12.698831

KEYWORDS: GRIN lenses, Objectives, Image quality, Tissue optics, Luminescence, Glasses, Monochromatic aberrations, Imaging systems, Tissues, Image resolution

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Proceedings Article | 12 February 2007 Paper

Paper

Multiphoton and magnetic resonance imaging of Barley embryos: comparing micro-imaging techniques across scale and parameter barriers

Martin Stark, Bertram Manz, Iris Riemann, Frank Volke, Winfriede Weschke, Karsten König

Proceedings Volume 6442, 644227 (2007) https://doi.org/10.1117/12.702364

KEYWORDS: Magnetic resonance imaging, Luminescence, Multiphoton microscopy, Magnetism, Objectives, Image segmentation, Absorption, Femtosecond phenomena, 3D image processing, Image resolution

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Proceedings Article | 10 February 2007 Paper

Paper

In-vivo multiphoton-endoscopy of endogenous skin fluorophores

Alexander Ehlers, Selma Schenkl, Iris Riemann, Bernhard Messerschmidt, Martin Kaatz, Rainer Bückle, Karsten König

Proceedings Volume 6442, 64421Y (2007) https://doi.org/10.1117/12.702394

KEYWORDS: Endoscopes, Skin, GRIN lenses, Second-harmonic generation, In vivo imaging, Collagen, Luminescence, Multiphoton tomography, Gradient-index optics, Tissue optics

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Proceedings Article | 10 February 2007 Paper

Paper

In vivo multiphoton tomography of skin during wound healing and scar formation

Iris Riemann, Alexander Ehlers, Ronan LeHarzic, Sven Martin, Annette Reif, Karsten König

Proceedings Volume 6442, 644226 (2007) https://doi.org/10.1117/12.702407

KEYWORDS: Skin, Tissues, In vivo imaging, Wound healing, Multiphoton tomography, Collagen, Near infrared, Optical fibers, Second-harmonic generation, Multiphoton microscopy

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Proceedings Article | 10 February 2007 Paper

Paper

The influence of NIR femtosecond laser radiation on the viability of 3D stem cell clusters and tumor spheroids

Aisada Uchugonova, Iris Riemann, Frank Stracke, Erwin Gorjup, Ronan LeHarzic, Karsten König

Proceedings Volume 6442, 64421Z (2007) https://doi.org/10.1117/12.702586

KEYWORDS: Stem cells, Femtosecond phenomena, Luminescence, Near infrared, Tumors, Pancreas, Nanoprocess, Microscopes, Oxygen, 3D image processing

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Proceedings Article | 10 February 2007 Paper

Paper

Optical nano-injection into cells and 3D stem cell clusters via a NIR femtosecond laser

Iris Riemann, Frank Stracke, Aisada Uchugonova, Sven Martin, Rainer Bückle, Karsten König

Proceedings Volume 6442, 64421F (2007) https://doi.org/10.1117/12.702399

KEYWORDS: Near infrared, Stem cells, Femtosecond phenomena, Luminescence, 3D acquisition, Pancreas, Tissues, Microscopes, Microscopy, Laser ablation

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Proceedings Article | 10 February 2007 Paper

Paper

Clinical in vivo two-photon microendoscopy for intradermal high-resolution imaging with GRIN optics

Karsten König, Alexander Ehlers, Iris Riemann, Selma Schenkl, Bernhard Messerschmidt, Rainer Bückle, Ronan Le Harzic, Peter Elsner, Martin Kaatz

Proceedings Volume 6442, 644215 (2007) https://doi.org/10.1117/12.702380

KEYWORDS: GRIN lenses, Collagen, In vivo imaging, Skin, Imaging systems, Femtosecond phenomena, Endoscopes, Multiphoton tomography, Gradient-index optics, Second-harmonic generation

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Proceedings Article | 7 March 2006 Paper

Paper

New developments in corneal refractive surgery with femtosecond laser pulses

Ronan Le Harzic, Sven Martin, Rainer Bückle, Christian Wüllner, Christof Donitzky, Iris Riemann, Karsten König

Proceedings Volume 6138, 61381J (2006) https://doi.org/10.1117/12.645802

KEYWORDS: Retina, Femtosecond phenomena, Objectives, Cornea, Laser therapeutics, Surgery, Laser vision correction, Eye, Near infrared, Laser safety

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Proceedings Article | 2 March 2006 Paper

Paper

High-resolution multiphoton optical tomography of tissues: an in vitro and in vivo study

Iris Riemann, Katja Schenke-Layland, Alexander Ehlers, Enrico Dimitrow, Martin Kaatz, Peter Elsner, Sven Martin, Karsten König

Proceedings Volume 6142, 61420N (2006) https://doi.org/10.1117/12.651358

KEYWORDS: Tissues, In vivo imaging, Collagen, Heart, Skin, Near infrared, Optical fibers, Second-harmonic generation, Optical tomography, In vitro testing

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Proceedings Article | 23 February 2006 Paper

Paper

In vivo multiphoton tomography of skin cancer

Karsten König, Iris Riemann, Alexander Ehlers, Rainer Buckle, Enrico Dimitrow, Martin Kaatz, Joachim Fluhr, Peter Elsner

Proceedings Volume 6089, 60890R (2006) https://doi.org/10.1117/12.646000

KEYWORDS: Tissues, Luminescence, Skin, Melanoma, In vivo imaging, Multiphoton tomography, Near infrared, Tissue optics, Second-harmonic generation, Fluorescence lifetime imaging

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Proceedings Article | 23 February 2006 Paper

Paper

Multiphoton imaging of corneal tissue with near-infrared femtosecond laser pulses: corneal optical tomography and its use in refractive surgery

Bao-Gui Wang, Karsten Koenig, Iris Riemann, Harald Schubert, Karl-Juergen Halbhuber

Proceedings Volume 6089, 60891M (2006) https://doi.org/10.1117/12.640594

KEYWORDS: Multiphoton microscopy, Laser therapeutics, Femtosecond phenomena, Tissues, Surgery, Optical tomography, Tissue optics, Laser vision correction, Second-harmonic generation, Microscopes

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Proceedings Article | 23 February 2006 Paper

Paper

Fluorescence lifetime imaging of human skin and hair

A. Ehlers, I. Riemann, T. Anhut, M. Kaatz, P. Elsner, K. König

Proceedings Volume 6089, 60890N (2006) https://doi.org/10.1117/12.645714

KEYWORDS: Skin, Fluorescence lifetime imaging, Luminescence, In vivo imaging, Collagen, Second-harmonic generation, Sensors, Tissues, Image resolution, Chemical analysis

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Proceedings Article | 23 February 2006 Paper

Paper

Multiphoton nanosurgery in cells and tissues

Iris Riemann, Frank Stracke, Daniel Sauer, Sven Martin, Karsten König

Proceedings Volume 6089, 608918 (2006) https://doi.org/10.1117/12.646096

KEYWORDS: Tissues, Near infrared, Femtosecond phenomena, Luminescence, Microscopes, Laser scanners, Biopsy, Multiphoton microscopy, Photons, Tissue optics

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Proceedings Article | 4 November 2005 Paper

Paper

Nanoprocessing of semiconductors and metals with nJ femtosecond laser pulses

R. Le Harzic, D. Sauer, I. Riemann, K. König

Proceedings Volume 5989, 59890O (2005) https://doi.org/10.1117/12.629659

KEYWORDS: Femtosecond phenomena, Microscopes, Nanoprocess, Metals, Laser ablation, Nanostructuring, Silicon, Semiconductors, Laser scanners, Objectives

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Proceedings Article | 28 October 2005 Paper

Paper

In-vivo non-invasive multiphoton tomography of human skin

Karsten König, Iris Riemann, Alexander Ehlers, Ronan Le Harzic

Proceedings Volume 5990, 59900W (2005) https://doi.org/10.1117/12.629658

KEYWORDS: Skin, Tissues, Luminescence, Tissue optics, In vivo imaging, Near infrared, Femtosecond phenomena, Second-harmonic generation, Ultraviolet radiation, Imaging systems

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High resolution non-invasive 3D imaging devices are required to detect pathogenic microorganisms such as Anthrax spores, bacteria, viruses, fungi and chemical agents entering biological tissues such as the epidermis. Due to the low light penetration depth and the biodamage potential, ultraviolet light sources can not be employed to realize intratissue imaging of bio- and chemohazards. We report on the novel near infrared laser technology multiphoton tomography and the high resolution 4D imaging tool DermaInspect for non-invasive detection of intratissue agents and their influence on cellular metabolism based on multiphoton autofluorescence imaging (MAI) and second harmonic generation (SHG). Femtosecond laser pulses in the spectral range of 750 nm to 850 nm have been used to image in vivo human skin with subcellular spatial and picosecond temporal resolution. The non-linear induced autofluorescence of both, skin tissues and microorganisms, originates mainly from naturally endogenous fluorophores/protein structures like NAD(P)H, flavins, keratin, collagen, elastin, porphyrins and melanin. Bacteria emit in the blue/green spectral range due to NAD(P)H and flavoproteins and, in certain cases, in the red spectral range due to the biosynthesis of Zn-porphyrins, coproporphyrin and protoporphyrin. Collagen and exogenous non-centrosymmetric molecules can be detected by SHG signals. The system DermaInspect consists of a wavelength-tunable compact 80/90 MHz Ti:sapphire laser, a scan module with galvo scan mirrors, piezo-driven objective, fast photon detector and time-resolved single photon counting unit. It can be used to perform optical sectioning and 3D autofluorescence lifetime imaging (τ-mapping) with 1 μm spatial resolution and 270 ps temporal resolution. The parameter fluorescence lifetime depends on the type of fluorophore and its microenvironment and can be used to distinguish bio- and chemohazards from cellular background and to gain information for pathogen identification. The novel in vivo non-invasive imaging system offers the possibility to detect and to localize CB agents in tissues and to gain information on their impact on respiratory chain activity, cell division and metabolism. The system DermaInspect can also be used to detect food and water contamination.

Proceedings Article | 14 October 2005 Paper

Paper

In-vivo corneal nonlinear optical tomography based on second harmonic and multiphoton autofluorescence imaging induced by near-infrared femtosecond lasers with rabbits

Baogui Wang, Karl-Juergen Halbhuber, Iris Riemann, Karsten Koenig

Proceedings Volume 5964, 59640O (2005) https://doi.org/10.1117/12.622204

KEYWORDS: Second-harmonic generation, Collagen, In vivo imaging, Visualization, Multiphoton microscopy, Femtosecond phenomena, Tissues, Optical tomography, Tomography, Cornea

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Proceedings Article | 8 October 2005 Paper

Paper

Nonlinear laser scanning microscopy and microprocessing of biological and technical materials using a new diode-pumped solid-state femtosecond laser with cavity dumping

Tiemo Anhut, Iris Riemann, Karsten Koenig, Ronan LeHarzic, Alexander Killi, Uwe Morgner

Proceedings Volume 5860, 58600I (2005) https://doi.org/10.1117/12.632948

KEYWORDS: Oscillators, Microscopes, Microscopy, Femtosecond phenomena, Laser scanners, Luminescence, Skin, Semiconductor lasers, Pulsed laser operation, Sapphire lasers

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Proceedings Article | 25 April 2005 Paper

Paper

In vivo multiphoton tomography of inflammatory tissue and melanoma

Iris Riemann, Enrico Dimitrow, Martin Kaatz, Joachim Fluhr, Peter Elsner, Jens Kobow, Karsten Konig

Proceedings Volume 5686, (2005) https://doi.org/10.1117/12.590533

KEYWORDS: Skin, In vivo imaging, Tissues, Melanoma, Near infrared, Biopsy, Luminescence, Diagnostics, Tissue optics, Optical tomography

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Proceedings Article | 25 April 2005 Paper

Paper

In vivo multiphoton tomography of skin as a tool to study the effects of topically applied probes and UV exposure

Iris Riemann, Alexander Ehlers, Annette Reif, Jens Kobow, Karsten Konig

Proceedings Volume 5686, (2005) https://doi.org/10.1117/12.590817

KEYWORDS: Skin, In vivo imaging, Tissues, Ultraviolet radiation, Near infrared, Confocal microscopy, Femtosecond phenomena, Tissue optics, Image resolution, Optical tomography

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Proceedings Article | 15 April 2005 Paper

Paper

In vivo animal follow-up studies on intrastromal surgery with near-infrared nanojoule femtosecond laser pulses

Baogui Wang, Iris Riemann, Karl-Juergen Halbhuber, Harald Schubert, Sigrun Kirste, Karsten Koenig

Proceedings Volume 5695, (2005) https://doi.org/10.1117/12.584193

KEYWORDS: Femtosecond phenomena, Laser therapeutics, Surgery, Laser ablation, Cornea, In vivo imaging, Laser tissue interaction, Wound healing, Near infrared, Laser vision correction

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Proceedings Article | 15 April 2005 Paper

Paper

Multiphoton nanosurgery in cells and tissues

Iris Riemann, Tiemo Anhut, Frank Stracke, Ronan Le Harzic, Karsten Koenig

Proceedings Volume 5695, (2005) https://doi.org/10.1117/12.590487

KEYWORDS: Tissues, Near infrared, Luminescence, Microscopes, Laser ablation, Femtosecond phenomena, Laser scanners, Multiphoton microscopy, Teeth, Image processing

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Proceedings Article | 12 April 2005 Paper

Paper

Time-resolved and spectrally resolved 5D multiphoton microscopy for analysis and nanoprocessing of materials

Karsten Konig, Iris Riemann, Herbert Schuck, Daniel Sauer, Thomas Velten, Ronan Le Harzic

Proceedings Volume 5713, (2005) https://doi.org/10.1117/12.590400

KEYWORDS: Silicon, Luminescence, Femtosecond phenomena, Semiconductor lasers, Microscopes, Nanoprocess, Near infrared, Nanostructuring, Spectral resolution, Polymers

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Proceedings Article | 30 March 2005 Paper

Paper

Multiphoton tomography of epidermis and dermis

Alexander Ehlers, Iris Riemann, Tiemo Anhut, Jens Kobow, Karsten Konig

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.590411

KEYWORDS: Skin, Imaging systems, Luminescence, Objectives, In vivo imaging, Second-harmonic generation, Tissues, GRIN lenses, Collagen, Pulsed laser operation

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Proceedings Article | 30 March 2005 Paper

Paper

Fluorescence lifetime images and correlation spectra obtained by multidimensional TCSPC

Wolfgang Becker, Axel Bergmann, Elke Haustein, Zdenek Petrasek, Petra Schwille, Christoph Biskup, Tiemo Anhut, Iris Riemann, Karsten Konig

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.588990

KEYWORDS: Luminescence, Fluorescence correlation spectroscopy, Fluorescence lifetime imaging, Sensors, Fluorescence resonance energy transfer, Microscopes, Proteins, Signal detection, Molecules, Picosecond phenomena

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SPIE Journal Paper | 1 March 2005 Open Access

Open Access

Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy

Katja Schenke-Layland, Iris Riemann, Ulrich Stock, Karsten König

JBO, Vol. 10, Issue 02, 024017, (March 2005) https://doi.org/10.1117/12.10.1117/1.1896966

KEYWORDS: Tissues, Collagen, Heart, Optical fibers, Second-harmonic generation, Femtosecond phenomena, Near infrared, Microscopy, Electron microscopy, Multiphoton microscopy

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Proceedings Article | 10 September 2004 Paper

Paper

Photodynamic therapy and knocking out of single tumor cells by multiphoton excitation processes

Iris Riemann, Peter Fischer, Karsten Koenig

Proceedings Volume 5462, (2004) https://doi.org/10.1117/12.545583

KEYWORDS: Tumors, Luminescence, Photodynamic therapy, Near infrared, Femtosecond phenomena, Tissue optics, Laser therapeutics, Oxygen, Multiphoton processes, Absorption

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Proceedings Article | 10 September 2004 Paper

Paper

Nanoprocessing of DNA with femtosecond laser

Karsten Koenig, Frank Garwe, Andrea Czaki, Gunther Maubach, Iris Riemann, Wolfgang Fritzsche

Proceedings Volume 5462, (2004) https://doi.org/10.1117/12.545730

KEYWORDS: Molecules, Nanoparticles, Femtosecond phenomena, Gold, Molecular lasers, Laser cutting, Particles, Silver, Atomic force microscopy, Near infrared

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Proceedings Article | 8 September 2004 Paper

Paper

Characterization of multiphoton laser scanning device optical parameters for image restoration

Frank Fischer, Karsten Konig, Stefan Puschmann, Roger Wepf, Iris Riemann, Volker Ulrich, Peter Fischer

Proceedings Volume 5463, (2004) https://doi.org/10.1117/12.545604

KEYWORDS: Point spread functions, Skin, Image restoration, Tissue optics, Laser scanners, Temporal resolution, Biopsy, Tissues, Microscopes, Luminescence

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Proceedings Article | 8 September 2004 Paper

Paper

Multiphoton imaging of cardiovascular structures

Katja Schenke-Layland, Florian Opitz, Iris Riemann, Ulrich Stock, Karsten Konig

Proceedings Volume 5463, (2004) https://doi.org/10.1117/12.545882

KEYWORDS: Heart, Tissues, Collagen, Near infrared, Blood vessels, Femtosecond phenomena, Imaging systems, Second-harmonic generation, Optical fibers, Arteries

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Proceedings Article | 8 September 2004 Paper

Paper

High-resolution multiphoton tomography of human skin in vivo and in vitro

Iris Riemann, Enrico Dimitrov, Peter Fischer, Annette Reif, Martin Kaatz, Peter Elsner, Karsten Konig

Proceedings Volume 5463, (2004) https://doi.org/10.1117/12.545561

KEYWORDS: Skin, In vivo imaging, Tissues, Femtosecond phenomena, Luminescence, Near infrared, Tissue optics, Biopsy, Imaging systems, Second-harmonic generation

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Proceedings Article | 13 July 2004 Paper

Paper

Noninvasive multiphoton imaging of cardiovascular structures using NIR femtosecond laser scanning microscopy

Katja Schenke-Layland, Iris Riemann, Ulrich Stock, Karsten Konig

Proceedings Volume 5312, (2004) https://doi.org/10.1117/12.528263

KEYWORDS: Collagen, Heart, Near infrared, Tissues, Femtosecond phenomena, Microscopy, Multiphoton microscopy, Optical fibers, Second-harmonic generation, Tissue optics

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Proceedings Article | 13 July 2004 Paper

Paper

Optical tomography of pigmented human skin biopsies

Iris Riemann, Peter Fischer, Martin Kaatz, Tobias Fischer, Peter Elsner, Enrico Dimitrov, Annette Reif, Karsten Konig

Proceedings Volume 5312, (2004) https://doi.org/10.1117/12.528278

KEYWORDS: Skin, Luminescence, Biopsy, Tissues, Tissue optics, Femtosecond phenomena, Near infrared, Picosecond phenomena, Imaging systems, Photons

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Proceedings Article | 13 July 2004 Paper

Paper

First in vivo animal studies on intraocular nanosurgery and multiphoton tomography with low-energy 80-MHz near-infrared femtosecond laser pulses

Karsten Konig, Bagui Wang, Oliver Krauss, Iris Riemann, Harald Schubert, Sigrun Kirste, Peter Fischer

Proceedings Volume 5314, (2004) https://doi.org/10.1117/12.528287

KEYWORDS: Femtosecond phenomena, Laser therapeutics, Laser ablation, Near infrared, Laser vision correction, Laser systems engineering, Collagen, Surgery, In vivo imaging, Tissue optics

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Proceedings Article | 21 June 2004 Paper

Paper

High-speed FLIM data acquisition by time-correlated single-photon counting

Wolfgang Becker, Axel Bergmann, Giovanni Biscotti, Karsten Koenig, Iris Riemann, Laimonas Kelbauskas, Christoph Biskup

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.529113

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Sensors, Microscopes, Fluorescence resonance energy transfer, Picosecond phenomena, Laser scanners, Molecules, Proteins, Signal detection

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Proceedings Article | 21 June 2004 Paper

Paper

Multiphoton FLIM and spectral imaging of cells and tissues

Karsten Konig, Iris Riemann, Gunter Ehrlich, Volker Ulrich, Peter Fischer

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.528499

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Fluorescence resonance energy transfer, Imaging spectroscopy, Microscopes, Picosecond phenomena, Tissues, Molecules, Femtosecond phenomena, Fluorescent proteins

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Proceedings Article | 10 July 2003 Paper

Paper

Optical gene transfer by femtosecond laser pulses

Karsten Konig, Iris Riemann, Uday Tirlapur

Proceedings Volume 4963, (2003) https://doi.org/10.1117/12.478020

KEYWORDS: Femtosecond phenomena, Luminescence, Near infrared, Laser therapeutics, Ultraviolet radiation, Green fluorescent protein, Absorption, Ionization, Microscopes, Single photon

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SPIE Journal Paper | 1 July 2003 Open Access

Open Access

High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution

Karsten Koenig, Iris Riemann

JBO, Vol. 8, Issue 03, (July 2003) https://doi.org/10.1117/12.10.1117/1.1577349

KEYWORDS: Luminescence, Skin, Tissues, In vivo imaging, Tissue optics, Second-harmonic generation, Femtosecond phenomena, Photons, Picosecond phenomena, Imaging systems

Proceedings Article | 17 June 2002 Paper

Paper

Optical tomography of human skin with subcellular spatial and picosecond time resolution using intense near infrared femtosecond laser pulses

Karsten Koenig, Uwe Wollina, Iris Riemann, Christiane Peukert, Karl-Juergen Halbhuber, Helga Konrad, Peter Fischer, Veronika Fuenfstueck, Tobias Fischer, Peter Elsner

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.470692

KEYWORDS: Skin, Luminescence, Tissues, Tissue optics, Femtosecond phenomena, Imaging systems, Near infrared, Biopsy, Picosecond phenomena, Image resolution

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Proceedings Article | 1 April 2002 Paper

Paper

Nanodissection of human chromosomes and ultraprecise eye surgery with nanojoule near-infrared femtosecond laser pulses

Karsten Koenig, Iris Riemann, Oliver Krauss, Wolfgang Fritzsche

Proceedings Volume 4633, (2002) https://doi.org/10.1117/12.461380

KEYWORDS: Femtosecond phenomena, Surgery, Nanoprocess, Tissues, Laser therapeutics, Brain, Tissue optics, Laser vision correction, Laser processing, Absorption

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Nanojoule and sub-nanojoule 80 MHz femtosecond laser pulses at 750-850 nm of a compact titanium:sapphire laser have been used for highly precise nanoprocessing of DNA as well as of intracellular and intratissue compartments. In particular, a mean power between 15 mW and 100 mW, 170 fs pulse width, submicron distance of illumination spots and microsecond beam dwell times on spots have been used for multiphoton- mediated nanoprocessing of human chromosomes, brain and ocular intrastromal tissue. By focusing the laser beam with high numerical aperture focusing optics of the laser scan system femt-O-cut and of modified multiphoton scanning microscopes to diffraction-limited spots and TW/cm² light intensities, precise submicron holes and cuts have been processed by single spot exposure and line scans. A minimum FWHM cut size below 70 nm during the partial dissection of the human chromosome 3 was achieved. Complete chromosome dissection could be performed with FWHM cut sizes below 200 nm. Intracellular chromosome dissection was possible. Intratissue processing in depths of 50 - 100micrometers and deeper with a precision of about 1micrometers including cuts through a nuclei of a single intratissue cell without destructive photo-disruption effects to surrounding tissue layers have been demonstrated in brain and eye tissues. The femt-O-cut system includes a diagnostic system for optical tomography with submicron resolution based on multiphoton- excited autofluorescence imaging (MAI) and second harmonic generation. This system was used to localize the intracellular and intratissue targets and to control the effects of nanoprocessing. These studies show, that in contrast to conventional approaches of material processing with amplified femtosecond laser systems and (mu) J pulse energies, nanoprocessing of materials including biotissues can be performed with nJ and sub-nJ high repetition femtosecond laser pulses of turn-key compact lasers without collateral damage. Potential applications include highly precise cell and embryo surgery, gene diagnostics and gene therapy, intrastromal refractive surgery, cancer therapy and brain surgery.

Proceedings Article | 18 December 2000 Paper

Paper

Multiphoton multicolor FISH

Karsten Koenig, Iris Riemann, Axel Goehlert, Peter Fischer, Thomas Liehr, Ivan Loncarevic, Uwe Claussen, Karl-Juergen Halbhuber

Proceedings Volume 4164, (2000) https://doi.org/10.1117/12.410640

KEYWORDS: Luminescence, Near infrared, Tissues, Femtosecond phenomena, Stereoscopy, Multiphoton microscopy, Fiber lasers, Microscopes, Microscopy, Pathology

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Proceedings Article | 19 July 1999 Paper

Paper

Intracellular nanosurgery with compact femtosecond laser

Karsten Koenig, Iris Riemann, Peter Fischer, Karl-Juergen Halbhuber

Proceedings Volume 3749, (1999) https://doi.org/10.1117/12.354799

KEYWORDS: Femtosecond phenomena, Laser therapeutics, Nanoprocess, Laser ablation, Near infrared, Laser development, Infrared radiation, Objectives, Laser scanners, Microscopes

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Proceedings Article | 6 July 1999 Paper

Paper

Photodynamic therapy by nonresonant two-photon excitation

Karsten Koenig, Iris Riemann, Peter Fischer

Proceedings Volume 3592, (1999) https://doi.org/10.1117/12.351512

KEYWORDS: Photodynamic therapy, Luminescence, Chlorine, Near infrared, Oxygen, Laser therapeutics, Microscopes, Pulsed laser operation, Femtosecond phenomena, Absorption

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Proceedings Article | 4 June 1999 Paper

Paper

Cloning assay thresholds on cells exposed to ultrafast laser pulses

Karsten Koenig, Iris Riemann, Peter Fischer, Thomas Becker, Hartmut Oehring, Karl-Juergen Halbhuber

Proceedings Volume 3616, (1999) https://doi.org/10.1117/12.351834

KEYWORDS: Microscopes, Picosecond phenomena, Luminescence, Femtosecond phenomena, Near infrared, Microscopy, Pulsed laser operation, Laser damage threshold, Argon ion lasers, Control systems

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Proceedings Article | 1 June 1999 Paper

Paper

Cloning assay thresholds on cells exposed to ultrafast laser pulses

Karsten Koenig, Iris Riemann, Peter Fischer, Thomas Becker, Hartmut Oehring, Karl-Juergen Halbhuber

Proceedings Volume 3604, (1999) https://doi.org/10.1117/12.349214

KEYWORDS: Microscopes, Picosecond phenomena, Luminescence, Near infrared, Microscopy, Pulsed laser operation, Femtosecond phenomena, Argon ion lasers, Laser damage threshold, Photonic microstructures

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Showing 5 of 58 publications

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