Surface plasmon resonance imaging optical biochips have shown promising applications in the field of
genetic diagnosis due to their ability to record hundred of different biomolecular interactions without any label
and in real time. Single nucleotide polymorphisms can be detected using this technique. To improve the accuracy
and the reliability of biochips a quantitative study on the impact of temperature on DNA hybridization and
secondary structures is shown for various oligonucleotides sequences. In this proceeding we focus on the impact
of temperature T on the binding rate kinetic τ, using the model case of DNA:DNA interactions. We show that
SPRI can quantify such characteristics as τ(T) and that they follow the Langmuir model variation law.
The present paper summarizes some of our work in the field of genetic diagnosis using Surface Plasmon Resonance
Imaging. The optical setup and its capability are presented, as well as the gold surface functionalization used. Results
obtained with oligonucleotides targets, specific to Cystic Fibrosis disease, in high and low concentration are shown. The
self-calibration method we have developed to reduce data dispersion in genetic diagnosis applications is described.
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