Matrix metalloproteinase 9 (MMP9) is present in normal physiological events in the human body. However, it is also known for being involved in inflammation process related to different diseases, such as arthritis and metastasis. Thus, this protein can be used as a biomarker for diseases monitoring and therapies control and assessments. The goal of this study is to demonstrate that novel multiplexed R-NPs optical transducers independent arrays, (also called BICELLs, Biological Sensing Cells) can discriminate small concentrations of MMP9. We achieved a LoD of 84 ng/mL. This data can be improved by increasing the resolution of the characterization system and optimizing the biofunctionalization strategy. These results are promising and encourage us to perform prospective works addressed to replicate this experiment using a fluidic system for multiplexed real-time optical response, so as to facilitate the monitoring of the different selected biomarkers (thank to multiplexed configuration of R-NPS arrays), necessary to provide patients with a more accurate diagnosis and treatments, and therefore to boost up personalized medicine.
Food allergy is a common disease worldwide with over 6% of the population (200-250 million people) suffering from any food allergy nowadays. The most dramatic increase seems to be happening in children and young people. Therefore, improvement in the diagnosis efficiency of these diseases are needed. Immunoglobulin type E (IgE) biomarker determination in human serum is a typical in vitro test for allergy identification. In this work we used a novel biosensor based on label-free photonic transducers called BICELLs (Biophotonic Sensing Cells) for IgE detection. These BICELLs have a thin film of nitrocellulose over the sensing surface, they can be vertical optically interrogated and are suitable for being integrated on-a-chip. The BICELLs sizes used were 100 and 800 µm of diameter. Calibration curves were obtained with IgE standards, by immobilizating anti-IgE antibodies and recognizing with standard IgE calibrators. The results, in similar assay format, were compared with commercial-available ImmunoCAP. The versatility of the interferometric nitrocellulose-based sensing surface was demonstrated since the limit of detections for BICELLs and ImmunoCAP were 0.7 and 0.35 kU/L, respectively.
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