Dr. Russell Edwin Connally Profile

Dr. Russell Edwin Connally

at Macquarie Univ

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Publications (8)

SPIE Journal Paper | 1 May 2008 Open Access

Solid-state time-gated luminescence microscope with ultraviolet light-emitting diode excitation and electron-multiplying charge-coupled device detection

Russell Connally, James Piper

JBO, Vol. 13, Issue 03, 034022, (May 2008) https://doi.org/10.1117/12.10.1117/1.2928169

KEYWORDS: Luminescence, Microscopes, Signal to noise ratio, Light emitting diodes, Cameras, Ultraviolet light emitting diodes, Sensors, Europium, Solid state electronics, Optical filters

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Proceedings Article | 25 October 2006 Paper

UV LED excited time-gated luminescence flow cytometry: concepts and experimental evaluation

Dayong Jin, Russell Connally, Jim Piper

Proceedings Volume 6380, 63800L (2006) https://doi.org/10.1117/12.685076

KEYWORDS: Luminescence, Flow cytometry, Target detection, Signal detection, Organisms, Ultraviolet light emitting diodes, Ultraviolet radiation, Sensors, Light emitting diodes, Photomultipliers

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Proceedings Article | 20 October 2006 Paper

A novel luminescence analyser for europium chelates using solid-state excitation and a gated photomultiplier

Russell Connally, Peter Dekker, James Piper

Proceedings Volume 6371, 63710K (2006) https://doi.org/10.1117/12.685691

KEYWORDS: Luminescence, Europium, Light emitting diodes, Photomultipliers, Lanthanides, Ocean optics, Switching, Ultraviolet light emitting diodes, Magnesium, Field effect transistors

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Proceedings Article | 4 April 2005 Paper

BHHST: An improved lanthanide chelate for time-resolved fluorescence applications

Russell Connally, Dayong Jin, James Piper

Proceedings Volume 5704, (2005) https://doi.org/10.1117/12.584818

KEYWORDS: Luminescence, Proteins, Signal to noise ratio, Europium, Time resolved spectroscopy, Lanthanides, Magnesium, Molecules, Microscopes, Statistical analysis

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Proceedings Article | 29 March 2005 Paper

Ultrasensitive time-resolved nanoliter volume fluorometry based on UV LEDs and a channel photomultiplier tube

Dayong Jin, Russell Connally, James Piper

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.590127

KEYWORDS: Ultraviolet light emitting diodes, Luminescence, Light emitting diodes, Ultraviolet radiation, Visible radiation, Europium, Molecules, Photomultipliers, Capillaries, Fluorometers

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SPIE Journal Paper | 1 July 2004 Open Access

Flash lamp-excited time-resolved fluorescence microscope suppresses autofluorescence in water concentrates to deliver an 11-fold increase in signal-to-noise ratio

Russell Connally, Duncan Veal, James Piper

JBO, Vol. 9, Issue 04, (July 2004) https://doi.org/10.1117/12.10.1117/1.1756594

KEYWORDS: Lamps, Luminescence, Microscopes, Bandpass filters, Plasma, Time resolved spectroscopy, Lanthanides, Cameras, Signal to noise ratio, Image intensifiers

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Proceedings Article | 12 September 2003 Paper

Time-resolvable fluorescent conjugates for the detection of pathogens in environmental samples containing autofluorescent material

Russell Connally, Duncan Veal, James Piper

Proceedings Volume 4967, (2003) https://doi.org/10.1117/12.478395

KEYWORDS: Luminescence, Proteins, Absorption, Pathogens, Microscopy, Europium, Environmental sensing, Molecules, Lanthanides, Time resolved spectroscopy

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Water is routinely monitored for environmental pathogens such a Cryptosporidium and Giardia using immunofluorescence microscopy (IFM). Autofluorescence can greatly diminish an operators capacity to resolve labeled pathogens from non-specific background. Naturally fluorescing components (autofluorophores) encountered in biological samples typically have fluorescent lifetimes (τ) of less than 100 nanoseconds and their emissions may be excluded through use of time-resolved fluorescence microscopy (TRFM). TRFM relies on the large differences in τ between autofluorescent molecules and long-lived lanthanide chelates. In TRFM, targets labeled with a time-resolvable fluorescent immunoconjugate are excited by an intense (UV) light pulse. A short delay is imposed to permit the decay of autofluorescence before capture of luminescence from the excited chelate using an image intensified CCD camera. In our experience, autofluorescence can be reduced to insignificant levels with a consequent 30-fold increase in target visibility using TRFM techniques. We report conjugation of a novel europium chelate to a monoclonal antibody specific for Giardia lamblia and use of the immunoconjugate for TRFM studies. Initial attempts to conjugate the same chelate to a monoclonal antibody directed against Cryptosporidium parvum led to poorly fluorescent constructs that were prone to denature and precipitate. We successfully conjugated BHHCT to anti-mouse polyvalent immunoglobulin and used this construct to overcome the difficulties in direct labeling of the anti-Cryptosporidium antibody. Both Giardia and Cryptosporidium were labeled using the anti-mouse protocol with a subsequent 20-fold and 6.6-fold suppression of autofluorescence respectively. A rapid protocol for conjugating and purifying the immunoconjugate was found and methods of quantifying the fluorescence to protein ratio determined. Performance of our TRFM was dependent on the quality and brightness of the immunoconjugate and optimization of the conjugation process is necessary to reap the full benefit of time-resolved techniques.

Proceedings Article | 9 July 2003 Paper

Novel flashlamp-based time-resolved fluorescence microscope reduces autofluorescence for 30-fold contrast enhancement in environmental samples

Russell Connally, Duncan Veal, James Piper

Proceedings Volume 4964, (2003) https://doi.org/10.1117/12.477977

KEYWORDS: Luminescence, Plasma, Cameras, Microscopes, Lanthanides, Image intensifiers, Time resolved spectroscopy, Microscopy, Quantum efficiency, Optical filters

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Showing 5 of 8 publications

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