Mud samples collected from bodies of water reveal information about the distribution of microorganisms in the local sediments. Hyperspectral imaging has been investigated as a technology to identify phototropic organisms living on sediments collected from the Texas Coastal Bend area based on their spectral pigment profiles and spatial arrangement. The top pigment profiles identified through high-performance liquid chromatography (HPLC) have been correlated with spectral signatures extracted from the hyperspectral data of mud using fast Fourier transform (FFT). Spatial distributions have also been investigated using 2D hyperspectral image processing. 2D pigment distribution maps have been created based on the correlation with pigment profiles in the FFT domain. Among the tested pigments, the results show match among four out of five pigment distribution trends between HPLC and hyperspectral data analysis. Differences are attributed mainly to the difference between area and volume of scale between the HPLC analysis and area covered by hyperspectral imaging.
KEYWORDS: Thermography, Digital photography, Hyperspectral imaging, Photography, Principal component analysis, Visualization, Temperature metrology, Cameras, Capacitance, Imaging systems
A comparative study of three imaging technologies has been conducted to nondestructively assess the thickness and strength of oyster shells grown in various environmental conditions. Oyster shell thickness and strength are expected to be dependent on the harshness of the oyster's environment as well as other factors. Oysters have been grown in environments with and without predators, and within and out of tidal zones. Hyperspectral imaging has been used to detect possible differences in hyperspectral properties among oyster shells from each of the four environments. Thermal Imaging has been utilized to identify hot spots in the shells based on the principles of heat capacitance, indicating density or thickness of the shells. Finally, a visible-range digital photographic camera has been used to obtain digital images. The three technologies are compared to evaluate the effectiveness of each technology in identifying oyster shell thickness and strength. Although oyster shell thickness and strength are related, they may not be exactly correlated. The local thickness of the oyster shells have been measured with a micro caliper, and shells broken with a crush tester to establish a baseline and ground truth for average shell thickness and shell strength, respectively. The preliminary results from the three methods demonstrate that thermal imaging correlates the best with the invasive strength test results and weight measurements. Using hyperspectral data and principal component analysis, classification of the four oyster shell groups were achieved. Visible-range images mainly provided size, shape, color and texture information.
Coral reefs are one of the most diverse and threatened ecosystems in the world. Corals worldwide are at risk, and in many instances, dying due to factors that affect their environment resulting in deteriorating environmental conditions. Because corals respond quickly to the quality of the environment that surrounds them, corals have been identified as bioindicators of water quality and marine environmental health. The hyperspectral imaging system is proposed as a noninvasive tool to monitor different species of corals as well as coral state over time. This in turn can be used as a quick and non-invasive method to monitor environmental health that can later be extended to climate conditions. In this project, a laboratory-based hyperspectral imaging system is used to collect spectral and spatial information of corals. In the work presented here, MATLAB and ENVI software tools are used to view and process spatial information and coral spectral signatures to identify differences among the coral data. The results support the hypothesis that hyperspectral properties of corals vary among different coral species, and coral state over time, and hyperspectral imaging can be a used as a tool to document changes in coral species and state.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.