Dual-wavelength excitation multispectral autofluorescence lifetime imaging handheld probe for label-free metabolic clinical imaging of the oral mucosa

Gabriel P. Tortorelli; Rodrigo Cuenca Martinez; Michael J. Serafino; Wihan Kim; Brian Applegate; Javier A. Jo

doi:10.1117/12.2608936

7 March 2022 Dual-wavelength excitation multispectral autofluorescence lifetime imaging handheld probe for label-free metabolic clinical imaging of the oral mucosa

Gabriel P. Tortorelli, Rodrigo Cuenca Martinez, Michael J. Serafino, Wihan Kim, Brian Applegate, Javier A. Jo

Author Affiliations +

Proceedings Volume PC11935, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2022; PC1193508 (2022) https://doi.org/10.1117/12.2608936
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Increased cellular metabolic activity, a hallmark of malignant epithelial cells, can be quantified by imaging the oral tissue autofluorescence originated from the metabolic cofactors NADH and FAD. We report a novel multispectral autofluorescence lifetime imaging (maFLIM) handheld probe capable of simultaneous autofluorescence excitation at 375 nm (for NADH) and 445 nm (for FAD), and simultaneous multispectral time-resolved fluorescence measurement at four emission spectral channels. The performance of the dual-wavelength excitation maFLIM handheld probe was assessed by imaging fluorescent dye standards with well characterized fluorescence lifetimes, and the oral mucosa of human subjects in oral health care settings.

Conference Presentation

Citation Download Citation

Gabriel P. Tortorelli, Rodrigo Cuenca Martinez, Michael J. Serafino, Wihan Kim, Brian Applegate, and Javier A. Jo "Dual-wavelength excitation multispectral autofluorescence lifetime imaging handheld probe for label-free metabolic clinical imaging of the oral mucosa", Proc. SPIE PC11935, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2022, PC1193508 (7 March 2022); https://doi.org/10.1117/12.2608936

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