Chemical imaging by fluorescence-detected photothermal mid-infrared (F-PTIR) microscopy

Garth J. Simpson

doi:10.1117/12.2610297

7 March 2022 Chemical imaging by fluorescence-detected photothermal mid-infrared (F-PTIR) microscopy

Garth J. Simpson

Proceedings Volume PC11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry; PC119570I (2022) https://doi.org/10.1117/12.2610297
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

F-PTIR is described theoretically and demonstrated experimentally for f high spatial resolution chemical microscopy based on mid-infrared absorption spectroscopy. In F-PTIR, heat produced locally by infrared absorption results in reductions in fluorescence through temperature-dependent changes in quantum efficiency. The point-spread function for F-PTIR is identical to that of fluorescence, simplifying the interpretation of image contrast relative to alternative scattering/refraction based photothermal detection methods. Fluorescence labeling enables infrared spectroscopy just in the regions associated with the label, providing dual selectivity for local chemical environments. F-PTIR spectroscopy was used to identify phase-separated domains within pharmaceutical materials impacting bioavailability and formulations design.

Conference Presentation

Citation Download Citation

Garth J. Simpson "Chemical imaging by fluorescence-detected photothermal mid-infrared (F-PTIR) microscopy", Proc. SPIE PC11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, PC119570I (7 March 2022); https://doi.org/10.1117/12.2610297

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