We applied multiphoton fluorescence, SHG, and SRS to investigate In situ and chemical-specific identification of lipid and mitochondrial composition is crucial in analyzing the impacted subcellular components resulting in lipid accumulation, and differentiate mitochondrial associated bimolecular identification and related lipids’ spatial distribution in skeletal muscle cells between athletes and type 2 diabetes patients, to reveal differences in composition related to dysregulated metabolic pathways. Furthermore, quantitative SRS analysis revealed markers of irregular molecules for impacted mitochondrial function corresponding to decreased lipid metabolism seen in type 2 diabetes.
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