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2 March 2022 Fluorescence detected Mid infrared photothermal microscopy
Yi Zhang, Ji-Xin Cheng, Haonan Zong, Cheng Zong, Meng Zhang, Yuying Tan, Yuewei Zhan, Danchen Jia
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Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730M (2022) https://doi.org/10.1117/12.2606441
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
We present a novel version of mid-infrared photothermal microscopy in which thermosensitive fluorescent probes are harnessed to sense the mid-infrared photothermal effect. The fluorescence intensity can be modulated at the level of 1% per Kelvin, which is 100 times larger than the modulation of scattering intensity. In addition, fluorescence emission is free of interference, thus much improving the image quality. Moreover, fluorophores can target specific organelles or biomolecules, thus augmenting the specificity of photothermal imaging. Spectral fidelity is confirmed by fingerprinting a single bacterium. A wide-field fluorescence-detected mid-infrared photothermal microscope developed allows video-rate bond-selective imaging of biological specimens.
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Yi Zhang, Ji-Xin Cheng, Haonan Zong, Cheng Zong, Meng Zhang, Yuying Tan, Yuewei Zhan, and Danchen Jia "Fluorescence detected Mid infrared photothermal microscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730M (2 March 2022); https://doi.org/10.1117/12.2606441
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KEYWORDS
Mid-IR
Luminescence
Microscopy
Fluorescence spectroscopy
Imaging spectroscopy
Infrared spectroscopy
Scattering
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