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Confocal fluorescence microscopy is a privileged tool for life imaging, but can generate phototoxicity due to the prolonged sample illumination. When cells are organized along sheets lying on 2D surfaces curved in a 3D volume (e.g. epithelial cells), we propose a new approach allowing to automatically estimate the surface on which these cells are distributed from a small number of acquisitions (typically 0.1% of the voxels). This allows to concentrate thereafter the illumination around the surface of interest and thus to scan only a small portion (typically between 1% and 5%) of the volume containing the sample.
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