Identifying metastatic melanoma with pump-probe microscopy of melanin

David Grass; Georgia M Beasley; Martin C. Fischer; M. Angelica Selim; Yue Zhou; Warren S. Warren

doi:10.1117/12.2655705

30 March 2023 Identifying metastatic melanoma with pump-probe microscopy of melanin

David Grass, Georgia M Beasley, Martin C. Fischer, M. Angelica Selim, Yue Zhou, Warren S. Warren

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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920F (2023) https://doi.org/10.1117/12.2655705
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

More patients diagnosed with stage I (local) melanoma die than any other metastatic stage, because there exists no biomarker to reliably diagnose metastatic melanoma, preventing many patients to receive appropriate treatment. We pursue an approach based on femtosecond pump-probe microscopy of melanin; a natural pigment found in most melanoma. The measured pump-probe signals of melanin are complex superpositions of multiple nonlinear processes, making interpretation challenging. We demonstrate how polarization control and data fitting are used to decompose melanin signals into their underlying nonlinear interactions. False colored images of a small set of melanoma tumors, based on specific nonlinear interactions, correlate with clinical concern. This approach of decomposing pump-probe signals is applicable to a multitude of different samples.

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Citation Download Citation

David Grass, Georgia M Beasley, Martin C. Fischer, M. Angelica Selim, Yue Zhou, and Warren S. Warren "Identifying metastatic melanoma with pump-probe microscopy of melanin", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920F (30 March 2023); https://doi.org/10.1117/12.2655705

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KEYWORDS

Melanoma

Microscopy

Diagnostics

Femtosecond phenomena

Nonlinear dynamics

Nonlinear optics

Polarization control

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