PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
We demonstrate optical redox ratio and fluorescence lifetime imaging microscopy (FLIM) of intrinsic metabolic co-factors NAD(P)H and FAD to quantify single cell metabolism and function. This approach is attractive because it does not require cell surface labels or transfection, enabling rapid assessment of dynamic events. Multiphoton microscopy provides near infrared excitation of these autofluorescent molecules, thereby maximizing cell viability. Newly trained neural networks automatically segment single cells for analysis of heterogeneity within and between patients. Overall, this approach is attractive for both basic research and patient management in cancer and immunology.
Melissa C. Skala
"Autofluorescence lifetime imaging of single cell metabolism", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285405 (13 March 2024); https://doi.org/10.1117/12.2685585
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Melissa C. Skala, "Autofluorescence lifetime imaging of single cell metabolism," Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285405 (13 March 2024); https://doi.org/10.1117/12.2685585