Bond-selective fluorescence imaging: from single-molecular to cellular imaging beyond video-rate

Dongkwan Lee; Haomin Wang; Lu Wei

doi:10.1117/12.3002679

13 March 2024 Bond-selective fluorescence imaging: from single-molecular to cellular imaging beyond video-rate

Dongkwan Lee, Haomin Wang, Lu Wei

Author Affiliations +

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550T (2024) https://doi.org/10.1117/12.3002679
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Bioimaging harnessing optical contrasts and chemical specificity is of vital importance in probing complex biology. Vibrational spectroscopy based on mid-infrared excitation can reveal rich chemical information about molecular distributions. However, its full potential for bioimaging is hindered by the achievable sensitivity. Here we report bond-selective fluorescence-detected infrared-excited (BonFIRE) spectro-microscopy. BonFIRE employs two-photon excitation in the mid- and near-infrared to upconvert vibrational excitations to electronic states for fluorescence detection, thus encoding vibrational information into fluorescence. We demonstrate BonFIRE spectral imaging in large spectroscopic windows on samples ranging from single molecules to biological specimens. We then extend BonFIRE imaging from point-scanning to wide-field configuration, enabling ultrafast imaging with a large field of view. We expect BonFIRE to expand the bioimaging toolbox by providing a new level of bond-specific vibrational information and facilitate biological investigations.

Conference Presentation

Citation Download Citation

Dongkwan Lee, Haomin Wang, and Lu Wei "Bond-selective fluorescence imaging: from single-molecular to cellular imaging beyond video-rate", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550T (13 March 2024); https://doi.org/10.1117/12.3002679

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KEYWORDS

Vibration

Fluorescence imaging

Biological imaging

Imaging spectroscopy

Spectroscopy

Picosecond phenomena

Single molecule spectroscopy

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