Monitoring the elasticity changes of HeLa cells during mitosis by atomic force microscopy

Ningcheng Jiang; Yuhua Wang; Jinshu Zeng; Xuemei Ding; Shusen Xie; Hongqin Yang

doi:10.1117/12.2245537

31 October 2016 Monitoring the elasticity changes of HeLa cells during mitosis by atomic force microscopy

Ningcheng Jiang, Yuhua Wang, Jinshu Zeng, Xuemei Ding, Shusen Xie, Hongqin Yang

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 1002427 (2016) https://doi.org/10.1117/12.2245537
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Cell mitosis plays a crucial role in cell life activity, which is one of the important phases in cell division cycle. During the mitosis, the cytoskeleton micro-structure of the cell changed and the biomechanical properties of the cell may vary depending upon different mitosis stages. In this study, the elasticity property of HeLa cells during mitosis was monitored by atomic force microscopy. Also, the actin filaments in different mitosis stages of the cells were observed by confocal imaging. Our results show that the cell in anaphase is stiffer than that in metaphase and telophase. Furthermore, lots of actin filaments gathered in cells’ center area in anaphase, which contributes to the rigidity of the cell in this phase. Our findings demonstrate that the nano-biomechanics of living cells could provide a new index for characterizing cell physiological states.

Citation Download Citation

Ningcheng Jiang, Yuhua Wang, Jinshu Zeng, Xuemei Ding, Shusen Xie, and Hongqin Yang "Monitoring the elasticity changes of HeLa cells during mitosis by atomic force microscopy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002427 (31 October 2016); https://doi.org/10.1117/12.2245537

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