Ultrahigh resolution optical coherence elastography combined with a rigid micro-endoscope (Conference Presentation)

Qi Fang; Andrea Curatolo; Philip Wijesinghe; Juliana Hamzah; Ruth Ganss; Peter B. Noble; Karol Karnowski; David D. Sampson; Jun Ki Kim; Wei M. Lee; Brendan F. Kennedy

doi:10.1117/12.2254815

19 April 2017 Ultrahigh resolution optical coherence elastography combined with a rigid micro-endoscope (Conference Presentation)

Qi Fang, Andrea Curatolo, Philip Wijesinghe, Juliana Hamzah, Ruth Ganss, Peter B. Noble, Karol Karnowski, David D. Sampson, Jun Ki Kim, Wei M. Lee, Brendan F. Kennedy

Author Affiliations +

Proceedings Volume 10053, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI; 1005309 (2017) https://doi.org/10.1117/12.2254815
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The mechanical forces that living cells experience represent an important framework in the determination of a range of intricate cellular functions and processes. Current insight into cell mechanics is typically provided by in vitro measurement systems; for example, atomic force microscopy (AFM) measurements are performed on cells in culture or, at best, on freshly excised tissue. Optical techniques, such as Brillouin microscopy and optical elastography, have been used for ex vivo and in situ imaging, recently achieving cellular-scale resolution. The utility of these techniques in cell mechanics lies in quick, three-dimensional and label-free mechanical imaging. Translation of these techniques toward minimally invasive in vivo imaging would provide unprecedented capabilities in tissue characterization. Here, we take the first steps along this path by incorporating a gradient-index micro-endoscope into an ultrahigh resolution optical elastography system. Using this endoscope, a lateral resolution of 2 µm is preserved over an extended depth-of-field of 80 µm, achieved by Bessel beam illumination. We demonstrate this combined system by imaging stiffness of a silicone phantom containing stiff inclusions and a freshly excised murine liver tissue. Additionally, we test this system on murine ribs in situ. We show that our approach can provide high quality extended depth-of-field images through an endoscope and has the potential to measure cell mechanics deep in tissue. Eventually, we believe this tool will be capable of studying biological processes and disease progression in vivo.

Conference Presentation

Citation Download Citation

Qi Fang, Andrea Curatolo, Philip Wijesinghe, Juliana Hamzah, Ruth Ganss, Peter B. Noble, Karol Karnowski, David D. Sampson, Jun Ki Kim, Wei M. Lee, and Brendan F. Kennedy "Ultrahigh resolution optical coherence elastography combined with a rigid micro-endoscope (Conference Presentation)", Proc. SPIE 10053, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI, 1005309 (19 April 2017); https://doi.org/10.1117/12.2254815

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Gradient-index optics

Tissue optics

Elastography

Tissues

Cell mechanics

Optical resolution

Coherence (optics)

Show All Keywords

Keywords/Phrases

Search In:

Publication Years