Three-dimensional imaging of micro-specimen by optical scanning holography

Jung-Ping Liu; Cheng-Hao Tsou

doi:10.1117/12.2269503

18 April 2017 Three-dimensional imaging of micro-specimen by optical scanning holography

Jung-Ping Liu, Cheng-Hao Tsou

Author Affiliations +

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510D (2017) https://doi.org/10.1117/12.2269503
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

Optical scanning holography (OSH) is a scanning-type digital holographic technique. In OSH, a heterodyne interference pattern is generated to raster scan the object. OSH can be operated in the incoherent mode and thus is able to record a fluorescence hologram. In addition, resolution of the OSH is proportional to the density of the interference pattern. Here we use a high-NA microscope objective to generate a dynamic Fresnel zone plate to record a hologram of micro-specimen. The achieved transverse resolution and longitudinal resolution are 0.78μm and 3.1μm, respectively.

Citation Download Citation

Jung-Ping Liu and Cheng-Hao Tsou "Three-dimensional imaging of micro-specimen by optical scanning holography", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510D (18 April 2017); https://doi.org/10.1117/12.2269503

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