Real-time three-dimensional counting and shape measurement of RBCs using digital holographic cytometry

Hideki Funamizu; Kotaro Sonoda; Ryoji Goto; Yoshihisa Aizu

doi:10.1117/12.2269425

18 April 2017 Real-time three-dimensional counting and shape measurement of RBCs using digital holographic cytometry

Hideki Funamizu, Kotaro Sonoda, Ryoji Goto, Yoshihisa Aizu

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 1025113 (2017) https://doi.org/10.1117/12.2269425
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

Digital holography is a useful technique for recording and reconstruction of the complex amplitude of an optical field. In this technique, an interference pattern of two waves is detected by an image sensor, and digital holograms are acquired in computer. The wavefront is reconstructed by a numerical calculation. In this study, we present the real-time threedimensional counting and shape measurement of RBCs using flow cytometry with digital holographic microscopy.

Citation Download Citation

Hideki Funamizu, Kotaro Sonoda, Ryoji Goto, and Yoshihisa Aizu "Real-time three-dimensional counting and shape measurement of RBCs using digital holographic cytometry", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 1025113 (18 April 2017); https://doi.org/10.1117/12.2269425

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