Multi-spectral endogenous fluorescence imaging for bacterial differentiation

Nikita V. Chernomyrdin; Margarita V. Babayants; Oleg V. Korotkov; Konstantin G. Kudrin; Elena N. Rimskaya; Irina A. Shikunova; Vladimir N. Kurlov; Olga P. Cherkasova; Gennady A. Komandin; Igor V. Reshetov; Kirill I. Zaytsev

doi:10.1117/12.2285065

28 July 2017 Multi-spectral endogenous fluorescence imaging for bacterial differentiation

Nikita V. Chernomyrdin, Margarita V. Babayants, Oleg V. Korotkov, Konstantin G. Kudrin, Elena N. Rimskaya, Irina A. Shikunova, Vladimir N. Kurlov, Olga P. Cherkasova, Gennady A. Komandin, Igor V. Reshetov, Kirill I. Zaytsev

Author Affiliations +

Proceedings Volume 10411, Clinical and Preclinical Optical Diagnostics; 104110B (2017) https://doi.org/10.1117/12.2285065
Event: European Conferences on Biomedical Optics, 2017, Munich, Germany

Abstract

In this paper, the multi-spectral endogenous fluorescence imaging was implemented for bacterial differentiation. The fluorescence imaging was performed using a digital camera equipped with a set of visual bandpass filters. Narrowband 365 nm ultraviolet radiation passed through a beam homogenizer was used to excite the sample fluorescence. In order to increase a signal-to-noise ratio and suppress a non-fluorescence background in images, the intensity of the UV excitation was modulated using a mechanical chopper. The principal components were introduced for differentiating the samples of bacteria based on the multi-spectral endogenous fluorescence images.

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Nikita V. Chernomyrdin, Margarita V. Babayants, Oleg V. Korotkov, Konstantin G. Kudrin, Elena N. Rimskaya, Irina A. Shikunova, Vladimir N. Kurlov, Olga P. Cherkasova, Gennady A. Komandin, Igor V. Reshetov, and Kirill I. Zaytsev "Multi-spectral endogenous fluorescence imaging for bacterial differentiation", Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics, 104110B (28 July 2017); https://doi.org/10.1117/12.2285065

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