Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy

Rafay Ahmed; Alan Wing Lun Law; Tsz Wing Cheung; Condon Lau

doi:10.1117/12.2283272

1 August 2017 Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy

Rafay Ahmed, Alan Wing Lun Law, Tsz Wing Cheung, Condon Lau

Author Affiliations +

Proceedings Volume 10417, Medical Laser Applications and Laser-Tissue Interactions VIII; 104170O (2017) https://doi.org/10.1117/12.2283272
Event: European Conferences on Biomedical Optics, 2017, Munich, Germany

Abstract

Subcritical calvarial defects are important to study bone regeneration during healing. In this study 1mm calvarial defects were created using trephine in the parietal bones of Sprague-Dawley rats (n=7) that served as in vivo defects. Subjects were sacrificed after 7 days and the additional defects were created on the harvested skull with the same method to serve as control defects. Raman spectroscopy is established to investigate mineral/matrix ratio, carbonate/phosphate ratio and crystallinity of three different surfaces; in vivo defects, control defects and normal surface. Results show 21% and 23% decrease in mineral/matrix after 7 days of healing from surface to in vivo and control to in vivo defects, respectively. Carbonate to phosphate ratio was found to be increased by 39% while crystallinity decreased by 26% in both surface to in vivo and control to in vivo defects. This model allows to study the regenerated bone without mechanically perturbing healing surface.

Citation Download Citation

Rafay Ahmed, Alan Wing Lun Law, Tsz Wing Cheung, and Condon Lau "Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy ", Proc. SPIE 10417, Medical Laser Applications and Laser-Tissue Interactions VIII, 104170O (1 August 2017); https://doi.org/10.1117/12.2283272

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