Particle tracking and extended object imaging by interferometric super resolution microscopy

Itay Gdor; Seunghwan Yoo; Xiaolei Wang; Matthew Daddysman; Rosemarie Wilton; Nicola Ferrier; Mark Hereld; Oliver (Ollie) Cossairt; Aggelos Katsaggelos; Norbert F. Scherer

doi:10.1117/12.2291245

23 February 2018 Particle tracking and extended object imaging by interferometric super resolution microscopy

Itay Gdor, Seunghwan Yoo, Xiaolei Wang, Matthew Daddysman, Rosemarie Wilton, Nicola Ferrier, Mark Hereld, Oliver (Ollie) Cossairt, Aggelos Katsaggelos, Norbert F. Scherer

Author Affiliations +

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 1049911 (2018) https://doi.org/10.1117/12.2291245
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.

Conference Presentation

Citation Download Citation

Itay Gdor, Seunghwan Yoo, Xiaolei Wang, Matthew Daddysman, Rosemarie Wilton, Nicola Ferrier, Mark Hereld, Oliver (Ollie) Cossairt, Aggelos Katsaggelos, and Norbert F. Scherer "Particle tracking and extended object imaging by interferometric super resolution microscopy", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 1049911 (23 February 2018); https://doi.org/10.1117/12.2291245

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