Quantitative evaluation of healing degree in injured tendons based on orientation analysis of collagen fibers by using Fourier-transform second-harmonic-generation microscopy and its relationship to mechanical property

Eiji Hase; Takeo Minamikawa; Katsuya Sato; Daisuke Yonekura; Mitsuhiko Takahashi; Takeshi Yasui

doi:10.1117/12.2318673

24 April 2018 Quantitative evaluation of healing degree in injured tendons based on orientation analysis of collagen fibers by using Fourier-transform second-harmonic-generation microscopy and its relationship to mechanical property

Eiji Hase, Takeo Minamikawa, Katsuya Sato, Daisuke Yonekura, Mitsuhiko Takahashi, Takeshi Yasui

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107110V (2018) https://doi.org/10.1117/12.2318673
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

We used Fourier-transform second-harmonic generation (FT-SHG) microscopy to analyze the orientation of collagen fibers in healing rabbit tendons recovered from an artificial transection. The histological difference between normal and healing tendons can be observed from normal SHG images, whereas the percentage of anisotropic (preferred orientation) regions obtained from the orientation analysis was well correlated (R² = 0.63) with Young’s modulus obtained from tensile testing of the same sample. Since Young’s modulus reflects the degree of mechanical healing, our results indicate that FT-SHG microscopy have a unique potential as a non-destructive and non-invasive modality for a simultaneous assessment of the histological and mechanical healing degree in injured tendons.

Citation Download Citation

Eiji Hase, Takeo Minamikawa, Katsuya Sato, Daisuke Yonekura, Mitsuhiko Takahashi, and Takeshi Yasui "Quantitative evaluation of healing degree in injured tendons based on orientation analysis of collagen fibers by using Fourier-transform second-harmonic-generation microscopy and its relationship to mechanical property", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107110V (24 April 2018); https://doi.org/10.1117/12.2318673

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Second-harmonic generation

Collagen

Microscopy

Image analysis

Tissues

Fourier transforms

Image processing

Keywords/Phrases

Search In:

Publication Years