Impacts of vascular diameter and depth on singlet oxygen luminescence imaging in tissue simulating phantom

Xianglian Liao; Yi Shen; Yunfei Yu; Huiyun Lin; Lisheng Lin; Ying Gu; Buhong Li

doi:10.1117/12.2502717

23 October 2018 Impacts of vascular diameter and depth on singlet oxygen luminescence imaging in tissue simulating phantom

Xianglian Liao, Yi Shen, Yunfei Yu, Huiyun Lin, Lisheng Lin, Ying Gu, Buhong Li

Author Affiliations +

Proceedings Volume 10820, Optics in Health Care and Biomedical Optics VIII; 108203I (2018) https://doi.org/10.1117/12.2502717
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

Singlet oxygen (¹O₂) is the primary cytotoxic production in type-II photodynamic therapy (PDT). The correlation between ¹O₂ generation and PDT efficacy during treatment has received considerable attention. The direct detection of ¹O₂ luminescence is the gold standard for ¹O₂ identification. However, the intensity of ¹O₂ luminescence could be influenced by tissue optical properties, location and morphology of lesion, especially for vascular targeting PDT. In this study, the impacts of vascular diameter and depth on ¹O₂ luminescence imaging have been investigated in tissue simulating phantom. A near-IR sensitive InGaAs camera with adaptive optics and CW laser 532 nm were used for fast imaging of ¹O₂ luminescence. Rose Bengal (RB) was used as photosensitizer to generate ¹O₂ during photosensitization. Intralipid was diluted with different concentrations in order to establish the scattering properties of tissues. The capillary tubes, containing solution of RB, with varied diameters from 0.1 to 0.9 mm were used to simulate the vascular with the depth varied from 0 to 5 mm in phantom. The preliminary results indicate that the profile range ratio of ¹O₂ luminescence images are negatively correlated to the diameter of capillary tube, and the attenuation of intensity of ¹O₂ luminescence is non-linear with the increase of depth.

Citation Download Citation

Xianglian Liao, Yi Shen, Yunfei Yu, Huiyun Lin, Lisheng Lin, Ying Gu, and Buhong Li "Impacts of vascular diameter and depth on singlet oxygen luminescence imaging in tissue simulating phantom", Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108203I (23 October 2018); https://doi.org/10.1117/12.2502717

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