Adaptive optics two-photon microscopy for in vivo imaging of cortex and hippocampus in mouse brain

Congping Chen; Zhongya Qin; Sicong He; Caleb Lui; Nancy Y. Ip; Jianan Y. Qu

doi:10.1117/12.2509784

1 March 2019 Adaptive optics two-photon microscopy for in vivo imaging of cortex and hippocampus in mouse brain

Congping Chen, Zhongya Qin, Sicong He, Caleb Lui, Nancy Y. Ip, Jianan Y. Qu

Proceedings Volume 10865, Neural Imaging and Sensing 2019; 108650M (2019) https://doi.org/10.1117/12.2509784
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Two-photon microscopy is a powerful tool for in vivo brain imaging that has greatly facilitated the neuroscience research in the past few decades. However, it still remains a challenge to image deep inside the brain with near diffraction-limited resolution due to the optical aberrations induced by the biological tissue and the cranial window. Here, we used an adaptive optics approach based on direct wavefront sensing to correct the aberration induced by the thinned skull window and achieved minimally invasive imaging of cerebral cortex with near-diffraction-limit resolution. Besides, by compensating the intrinsic aberration of a miniature gradient-index lens that implanted into the brain, two-photon imaging of hippocampal dendritic spines was realized over an extended field of view. The improvement in fluorescence intensity and imaging resolution enabled us to resolve the fine structures in live mouse brain such as dendritic spines that were invisible without the help of adaptive optics.

Conference Presentation

Citation Download Citation

Congping Chen, Zhongya Qin, Sicong He, Caleb Lui, Nancy Y. Ip, and Jianan Y. Qu "Adaptive optics two-photon microscopy for in vivo imaging of cortex and hippocampus in mouse brain", Proc. SPIE 10865, Neural Imaging and Sensing 2019, 108650M (1 March 2019); https://doi.org/10.1117/12.2509784

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available