Free electron mediated effects of femtosecond pulse series in the (irradiance/irradiation dose) parameter space (Conference Presentation)

Norbert Linz; Xiao-Xuan Liang; Sebastian Freidank; Alfred Vogel

doi:10.1117/12.2525153

8 March 2019 Free electron mediated effects of femtosecond pulse series in the (irradiance/irradiation dose) parameter space (Conference Presentation)

Norbert Linz, Xiao-Xuan Liang, Sebastian Freidank, Alfred Vogel

Author Affiliations +

Proceedings Volume 10876, Optical Interactions with Tissue and Cells XXX; 1087612 (2019) https://doi.org/10.1117/12.2525153
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Understanding free-electron mediated effects of tightly focused femtosecond pulse series is essential for minimizing photodamage in nonlinear microscopy and opens new avenues for nanosurgery and intentional modifications of biomolecules. We tracked different stages of the photomodification kinetics (hyperfluorescence, plasma luminescence, bubble formation) by time-lapse 2-photon microscopy, fluorescence lifetime measurements, and bubble interferometry with nanometer resolution. Monitoring of bubble growth during pulse series enabled us to quantify chemical reaction rates leading to gas formation via molecular disintegration. Novel ways of data evaluation were used to create a comprehensive picture of the photomodification kinetics in the (irradiance/irradiation dose) parameter space.

Conference Presentation

Citation Download Citation

Norbert Linz, Xiao-Xuan Liang, Sebastian Freidank, and Alfred Vogel "Free electron mediated effects of femtosecond pulse series in the (irradiance/irradiation dose) parameter space (Conference Presentation)", Proc. SPIE 10876, Optical Interactions with Tissue and Cells XXX, 1087612 (8 March 2019); https://doi.org/10.1117/12.2525153

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