Cell trauma detection using infra-red live cell imaging

Ben O. L. Mellors; Hamid Dehghani; Christopher R. Howle; Abigail M. Spear

doi:10.1117/12.2525012

19 July 2019 Cell trauma detection using infra-red live cell imaging

Ben O. L. Mellors, Hamid Dehghani, Christopher R. Howle, Abigail M. Spear

Proceedings Volume 11073, Clinical and Preclinical Optical Diagnostics II; 110730M (2019) https://doi.org/10.1117/12.2525012
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Infra-red (IR) spectroscopic imaging of live cells is greatly affected by the presence of water, which is a strong absorber of IR radiation. In order to overcome this, a variety of methods have been developed using complex microfluidic devices to reduce the liquid sample path length. However, these devices are often custom made needing both specialised equipment and detailed fabrication steps. Here we show the novel utilisation of a liquid-air interface configuration and a negative contrast imaging device (NCI) reflectance imaging system for the collection of spectral data from live cells within an in vitro environment. Spectral differences were observed between two different cell densities, both in the presence and absence of cell culture media. Additionally, differences were observed between control and test cultures exposed to dimethyl sulfoxide (DMSO) to induce cell apoptosis. The NCI system acquired data in the 2.5 – 3.5 μm spectral region, at a spectral sampling interval of 10 nm. This method will allow further investigation of spectral biomarkers within cell cultures to augment understanding of specific cell contributions to wound healing in vivo.

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Ben O. L. Mellors, Hamid Dehghani, Christopher R. Howle, and Abigail M. Spear "Cell trauma detection using infra-red live cell imaging", Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110730M (19 July 2019); https://doi.org/10.1117/12.2525012

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KEYWORDS

Live cell imaging

Infrared imaging

Cell death

FT-IR spectroscopy

Infrared spectroscopy

Interfaces

Mid-IR

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