Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

Pavel V. Shalaev; Ekaterina V. Bondina; Sergey A. Dolgushin; Victoria S. Semeykina; Natalya N. Sankova; Ekaterina V. Parkhomchuk

doi:10.1117/12.2526387

19 July 2019 Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

Pavel V. Shalaev, Ekaterina V. Bondina, Sergey A. Dolgushin, Victoria S. Semeykina, Natalya N. Sankova, Ekaterina V. Parkhomchuk

Author Affiliations +

Proceedings Volume 11073, Clinical and Preclinical Optical Diagnostics II; 110731W (2019) https://doi.org/10.1117/12.2526387
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

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Pavel V. Shalaev, Ekaterina V. Bondina, Sergey A. Dolgushin, Victoria S. Semeykina, Natalya N. Sankova, and Ekaterina V. Parkhomchuk "Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis", Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110731W (19 July 2019); https://doi.org/10.1117/12.2526387

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