Holographic imaging for 3D cells morphology in microfluidic flow

Pasquale Memmolo; Massimiliano Villone; Francesco Merola; Martina Mugnano; Lisa Miccio; Pier Luca Maffettone; Pietro Ferraro

doi:10.1117/12.2527129

22 July 2019 Holographic imaging for 3D cells morphology in microfluidic flow

Pasquale Memmolo, Massimiliano Villone, Francesco Merola, Martina Mugnano, Lisa Miccio, Pier Luca Maffettone, Pietro Ferraro

Author Affiliations +

Proceedings Volume 11076, Advances in Microscopic Imaging II; 110761U (2019) https://doi.org/10.1117/12.2527129
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

The complete cells characterization in microfluidic flow can be achieved by using the quantitative phase imaging by digital holography as imaging tool. In fact, by assuring the complete 3D rotation of flowing cells, it is possible to recover their 3D refractive index mapping by using the tomographic phase-contrast reconstruction. In this paper, we investigate all steps need to obtain the tomographic reconstruction of flowing cells. In particular, we employ a holographic 3D tracking algorithm to follow each cells that moves in the field of view, along with a suitable tracking angle method for the cell’s tumbling. Moreover, a fluid modeling is used to characterize the cell rotation effect. We test the proposed processing pipeline for circulating tumor cells.

Citation Download Citation

Pasquale Memmolo, Massimiliano Villone, Francesco Merola, Martina Mugnano, Lisa Miccio, Pier Luca Maffettone, and Pietro Ferraro "Holographic imaging for 3D cells morphology in microfluidic flow", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110761U (22 July 2019); https://doi.org/10.1117/12.2527129

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