Imaging characteristic optimization of digital holographic microscopy for onion epidermal cells

Rikang Qin; Yanyan Jia; Yiwei Liu; Hongpeng Qin; Yibo Wang; Zhuqing Jiang

doi:10.1117/12.2537550

18 November 2019 Imaging characteristic optimization of digital holographic microscopy for onion epidermal cells

Rikang Qin, Yanyan Jia, Yiwei Liu, Hongpeng Qin, Yibo Wang, Zhuqing Jiang

Proceedings Volume 11188, Holography, Diffractive Optics, and Applications IX; 111881W (2019) https://doi.org/10.1117/12.2537550
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

The optimization of image resolution for digital holographic scanning imaging of biological cells is investigated. Digital holographic scanning imaging experiments on the upper epidermal cells of onions are performed to demonstrate the validity of resolution optimization algorithm. In the experiments, the holograms of the upper epidermal cells of onion are recorded at a certain scanning rate, and then are processed by using the resolution optimization algorithm. As a result, the phase images of the onion epidermal cells with higher contrast and resolution are obtained. According to the synthetic holograms, the changes of cell nucleus and actin microfilament inside onion’s epidermal cells are displayed. In addition, the dehydration process and plasmolysis phenomenon inside onion epidermal cells are also exhibited by recording longterm scanning holograms of living epidermal cells. The experimental results demonstrate that image quality of living onion epidermal cells can be improved by optimizing the algorithms.

Citation Download Citation

Rikang Qin, Yanyan Jia, Yiwei Liu, Hongpeng Qin, Yibo Wang, and Zhuqing Jiang "Imaging characteristic optimization of digital holographic microscopy for onion epidermal cells", Proc. SPIE 11188, Holography, Diffractive Optics, and Applications IX, 111881W (18 November 2019); https://doi.org/10.1117/12.2537550

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