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10 March 2020 Rapid antibiotic susceptibility testing by single bacterium stimulated Raman metabolic imaging (Conference Presentation)
Ji-Xin Cheng
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Proceedings Volume 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020; 112230A (2020) https://doi.org/10.1117/12.2543424
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
The widespread use of antibiotics has significantly increased the number of resistant bacteria, which has also increased the urgency of rapid bacterial detection and profiling their antibiotic response. Current clinical methods for antibiotic susceptibility testing (AST) rely on culture and require at least 16 to 24 h to conduct. We demonstrate a rapid AST method by monitoring the glucose and/or D2O metabolic activity of live bacteria at the single cell level with hyperspectral stimulated Raman scattering (SRS) imaging.
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Ji-Xin Cheng "Rapid antibiotic susceptibility testing by single bacterium stimulated Raman metabolic imaging (Conference Presentation)", Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 112230A (10 March 2020); https://doi.org/10.1117/12.2543424
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KEYWORDS
Bacteria
Raman spectroscopy
Glucose
Hyperspectral imaging
Profiling
Raman scattering
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