Towards combined multispectral, FLIM and Raman imaging for skin diagnostics

J. Spigulis; I. Kuzmina; V. Lukinsone; M. Tamošiūnas; I. Oshina; L. Ozolina; A. Maslobojeva; M. Kuzminskis; D. Ivanov; E. Borisova

doi:10.1117/12.2559107

17 February 2020 Towards combined multispectral, FLIM and Raman imaging for skin diagnostics

J. Spigulis, I. Kuzmina, V. Lukinsone, M. Tamošiūnas, I. Oshina, L. Ozolina, A. Maslobojeva, M. Kuzminskis, D. Ivanov, E. Borisova

Author Affiliations +

Proceedings Volume 11232, Multimodal Biomedical Imaging XV; 112320N (2020) https://doi.org/10.1117/12.2559107
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

To explore challenges for further improvement of diagnostic performance, a project aimed at development of technology for tri-modal skin imaging by combining multispectral, fluorescence lifetime and Raman band imaging was initiated. In this study, each of the mentioned imaging modalities has been preliminary tested and updated. Four different multispectral imaging devices were tested on color standards. Picosecond laser-excited fluorescence lifetime imaging equipment was examined on ex-vivo skin samples. Finally, a new Raman spectroscopy setup with 785 nm laser was launched and tested on cell cultures and ex-vivo skin. Advantages and specific features of the tri-modal skin imaging are discussed.

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J. Spigulis, I. Kuzmina, V. Lukinsone, M. Tamošiūnas, I. Oshina, L. Ozolina, A. Maslobojeva, M. Kuzminskis, D. Ivanov, and E. Borisova "Towards combined multispectral, FLIM and Raman imaging for skin diagnostics", Proc. SPIE 11232, Multimodal Biomedical Imaging XV, 112320N (17 February 2020); https://doi.org/10.1117/12.2559107

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