Sensing of diseased mitochondria proportion by DEP at the organelle level of intact cells

Pei-Yin Chi; Ting-Wei Chuang; Tzu-Tsai Chu; Chia-Tzu Kuo; Yu-Ting Wu; Vahid Farmehini; Dar-Bin Shieh M.D.; Fan-Gang Tseng; Yau-Huei Wei; Nathan Swami; Chia-Fu Chou

doi:10.1117/12.2540653

21 February 2020 Sensing of diseased mitochondria proportion by DEP at the organelle level of intact cells

Pei-Yin Chi, Ting-Wei Chuang, Tzu-Tsai Chu, Chia-Tzu Kuo, Yu-Ting Wu, Vahid Farmehini, Dar-Bin Shieh M.D., Fan-Gang Tseng, Yau-Huei Wei, Nathan Swami, Chia-Fu Chou

Author Affiliations +

Proceedings Volume 11235, Microfluidics, BioMEMS, and Medical Microsystems XVIII; 112350N (2020) https://doi.org/10.1117/12.2540653
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

To monitor the healthiness of cells, it is very important to develop quick ways to characterize cells with the diseased and normal mitochondria. Thus, we can take measures to prevent potential development of mitochondria-related diseases. Physical study and systemic proof of concept by engineering method to perform quick diagnosis of the normal and diseased mitochondria have not been well achieved yet. In this study, we used insulator dielectrophoresis (i-DEP), by utilizing the intrinsic dielectric response of a cell to an external AC electric field to differentiate cells containing partially diseased and normal mitochondria to test if we can select diseased mitochondria-containing cells but not the normal cells. We found that it is possible to detect the diseased and healthy mitochondria in cells without breaking the cell membrane. Taking advantage of the dielectrophoresis, DEP principle, we may develop a quick diagnostic method to detect diseased mitochondria in living cells.

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Citation Download Citation

Pei-Yin Chi, Ting-Wei Chuang, Tzu-Tsai Chu, Chia-Tzu Kuo, Yu-Ting Wu, Vahid Farmehini, Dar-Bin Shieh M.D., Fan-Gang Tseng, Yau-Huei Wei, Nathan Swami, and Chia-Fu Chou "Sensing of diseased mitochondria proportion by DEP at the organelle level of intact cells", Proc. SPIE 11235, Microfluidics, BioMEMS, and Medical Microsystems XVIII, 112350N (21 February 2020); https://doi.org/10.1117/12.2540653

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