Second harmonic generation imaging collagen structure modulation in embryonic chicken cornea

Sheng-Lin Lee; Yang-Fang Chen; Chen-Yuan Dong

doi:10.1117/12.2545602

20 February 2020 Second harmonic generation imaging collagen structure modulation in embryonic chicken cornea

Sheng-Lin Lee, Yang-Fang Chen, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 1124414 (2020) https://doi.org/10.1117/12.2545602
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Most of the cornea is organized through a lattice pattern of collagen fibrils which is responsible for its transparency. Developing avian cornea is a highly organized extracellular matrix composed largely of striated collagen fibrils. In this study, we use embryonic corneas from chicks as model animal to study the temporal and spatial variation of corneal stroma. Through the use of Fast Fourier Transform second harmonic generation microscopy, we quantified collagen alignment of the entire corneal thickness during development. Corneal structural variation at different stage of developing embryos was studied. These results indicate that both the rotational pitch and overall rotational angle of corneal stroma is highly involved in the developing process of cornea, and these properties exhibit strong correlation during the development of left and right corneas.

Citation Download Citation

Sheng-Lin Lee, Yang-Fang Chen, and Chen-Yuan Dong "Second harmonic generation imaging collagen structure modulation in embryonic chicken cornea", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124414 (20 February 2020); https://doi.org/10.1117/12.2545602

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