From multi-photon absorption and stimulated emission to forbidden S0→T1 transition (Conference Presentation)

Zygmunt K. Gryczynski; Ignacy Gryczynski

doi:10.1117/12.2551828

9 March 2020 From multi-photon absorption and stimulated emission to forbidden S0→T1 transition (Conference Presentation)

Zygmunt K. Gryczynski, Ignacy Gryczynski

Author Affiliations +

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112441I (2020) https://doi.org/10.1117/12.2551828
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We will overview our initial developments of two, three, and four-photon excitation that started in early nineties with parallel developments in two-color two photon excitation to applications of light quenching (light stimulated emission) as a process to shape the excited state population. We will summarize our basic spectroscopy and time-resolved work in a cuvette system to total internal reflection applications. We will present more recent application of multi-photon processes in the case of metal enhanced fluorescence (MAF) and surface plasmons coupled emission (SPCE). Finally we will present the most recent observation of directly excited phosphorescence (S0→T1 excitation) and discuss potential applications in protein studies and cellular imaging.

Conference Presentation

Citation Download Citation

Zygmunt K. Gryczynski and Ignacy Gryczynski "From multi-photon absorption and stimulated emission to forbidden S0→T1 transition (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441I (9 March 2020); https://doi.org/10.1117/12.2551828

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