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9 March 2020 3D fluorescence deconvolution with deep priors (Conference Presentation)
Kevin Zhang, Michael R. Kellman, Emrah Bostan, Laura Waller
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Proceedings Volume 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII; 112450N (2020) https://doi.org/10.1117/12.2545041
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
The goal of this work is to incorporate Convolutional Neural Networks (CNNs) into the 3D deconvolution process without training. CNNs are well suited to the problem of 2D deconvolution, however training a CNN on 3D volumes requires excessive time and impractical amounts of training data. To circumvent these problems, we use a CNN architecture as if it were a handcrafted prior, similar to the work deep image prior. Using this method, we achieve high SSIM and PSNR metrics relative to other modern techniques for deconvolving through-focus fluorescence measurements to recover a 3D volume with no training data and minimal hyperparameter tuning.
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Kevin Zhang, Michael R. Kellman, Emrah Bostan, and Laura Waller "3D fluorescence deconvolution with deep priors (Conference Presentation)", Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 112450N (9 March 2020); https://doi.org/10.1117/12.2545041
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KEYWORDS
Deconvolution
Luminescence
Computer vision technology
Convolutional neural networks
Machine learning
Machine vision
Microscopes
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