High-resolution vascular imaging of small animal using the NIR-IIb window emitted from ICG

Meishan Cai; Zhenhua Hu; Jie Tian

doi:10.1117/12.2547582

16 March 2020 High-resolution vascular imaging of small animal using the NIR-IIb window emitted from ICG

Meishan Cai, Zhenhua Hu, Jie Tian

Proceedings Volume 11312, Medical Imaging 2020: Physics of Medical Imaging; 113122F (2020) https://doi.org/10.1117/12.2547582
Event: SPIE Medical Imaging, 2020, Houston, Texas, United States

Abstract

Fluorescence molecular imaging is a promising tool for molecular tracking, and thus can visualize the vascular structure of small animal. However, with the strong scattering in biological tissues, traditional fluorescence molecular imaging in the visible spectrum or the first near-infrared spectrum (NIR-I, 700-900 nm) has a limitation for high-resolution vascular imaging. Recently, the novel in vivo fluorescence molecular imaging in the longer second near-infrared window (NIR-IIb, 1500-1700 nm) is successfully developed for small animal imaging. The NIR-IIb window affords high imaging resolution and deep tissue penetration because of the diminished photon scattering effect, which is suitable for vascular imaging. However, the clinical applications of NIR-IIb fluorescence molecular imaging have been severely limited for lack of the clinical fluorophores. Here, we show that the clinically available dye, indocyanine green (ICG), can also emit the NIR-IIb signal, which provides high-resolution vascular imaging of small animal. We construct a novel imaging system for NIR-I and NIR-IIb imaging simultaneously and perform two vascular imaging experiments. The results demonstrate that the NIR-IIb imaging using ICG shows great superiority for high-resolution vascular imaging of small animal compared with NIR-I imaging. It is believed that this study will facilitate the preclinical and clinical applications of NIR-IIb molecular imaging using ICG in the future.

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Meishan Cai, Zhenhua Hu, and Jie Tian "High-resolution vascular imaging of small animal using the NIR-IIb window emitted from ICG", Proc. SPIE 11312, Medical Imaging 2020: Physics of Medical Imaging, 113122F (16 March 2020); https://doi.org/10.1117/12.2547582

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KEYWORDS

Vascular imaging

Molecular imaging

Luminescence

Imaging systems

Brain

Image resolution

Scattering

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