Multiline illumination Raman microscopy for rapid cell imaging

Kentaro Mochizuki; Yasuaki Kumamoto; Katsumasa Fujita

doi:10.1117/12.2573222

15 June 2020 Multiline illumination Raman microscopy for rapid cell imaging

Kentaro Mochizuki, Yasuaki Kumamoto, Katsumasa Fujita

Author Affiliations +

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 115210F (2020) https://doi.org/10.1117/12.2573222
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

Acceleration of image acquisition rate in Raman microscopy has been required to fully utilize its analytical advantages for biological/medical applications. By introducing the multiple line illumination and parallel spectral detection capability, image acquisition rate in the Raman microscope was improved < 104 times, compared with the conventional confocal Raman. High-resolution spontaneous Raman imaging of cells/tissues was thus enabled with an image acquisition time of a few minutes. Subsequent high-throughput Raman imaging-based analyses were also performed, including multiplex Raman tag imaging, cell classification, microplastic detection.

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Kentaro Mochizuki, Yasuaki Kumamoto, and Katsumasa Fujita "Multiline illumination Raman microscopy for rapid cell imaging", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 115210F (15 June 2020); https://doi.org/10.1117/12.2573222

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