Whole body fluorescence lifetime multiplexing of tumor receptor expression

Rahul Pal; Homan Kang; Hak Soo Choi; Gabriel D. Duda; Anand T. N. Kumar

doi:10.1117/12.2578412

5 March 2021 Whole body fluorescence lifetime multiplexing of tumor receptor expression

Rahul Pal, Homan Kang, Hak Soo Choi, Gabriel D. Duda, Anand T. N. Kumar

Proceedings Volume 11624, Visualizing and Quantifying Drug Distribution in Tissue V; 1162408 (2021) https://doi.org/10.1117/12.2578412
Event: SPIE BiOS, 2021, Online Only

Abstract

Fluorescence imaging of cancers using continuous wave (CW) detection of receptor targeted probes offer poor sensitivity and specificity due to background autofluorescence and non-specific probe accumulation. Here we show that fluorescence lifetime (FLT) imaging can significantly improve tumor contrast using epidermal growth factor receptor (EGFR) and programed death ligand 1 (PD-L1) targeted probes in a preclinical model of human breast cancer. Our results suggest that these probes have significantly longer FLTs in tumors than in normal tissue and the FLT enhancement is receptor dependent. We also show potential for simultaneous quantification of EGFR and PD-L1 using in vivo FLT multiplexing.

Conference Presentation

Citation Download Citation

Rahul Pal, Homan Kang, Hak Soo Choi, Gabriel D. Duda, and Anand T. N. Kumar "Whole body fluorescence lifetime multiplexing of tumor receptor expression", Proc. SPIE 11624, Visualizing and Quantifying Drug Distribution in Tissue V, 1162408 (5 March 2021); https://doi.org/10.1117/12.2578412

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