Jindou Shi,1,2 Prabuddha Mukherjee,2,1 Aneesh Alex,3,2 Edita Aksamitiene,2,4 Darold Spillman Jr.https://orcid.org/0000-0001-9946-2659,1,2 Marina Marjanovic,5,2 Minh Doan,3 Steve R. Hood,6,2 Stephen A. Boppart1,2,7
1Beckman Institute for Advanced Science and Technology (United States) 2GSK Ctr. for Optical Molecular Imaging (United States) 3GlaxoSmithKline (United States) 4Beckman Institute for Advanced Science and Technology, Univ. of Illinois (United States) 5Carle Illinois College of Medicine, Univ. of Illinois (United States) 6GlaxoSmithKline (United Kingdom) 7Univ. of Illinois (United States)
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An efficient and automated image analysis pipeline is essential for extracting quantitative information from multimodal image datasets. In this study, a multimodal optical imaging platform was used to capture CARS, 2PF, and FLIM images from control and drug-treated cells. Images were collected using both fluorescent label-based and label-free approaches. Here we present a single-cell analysis pipeline for the multimodal cellular image analysis. The results demonstrate the capability of our single-cell analysis pipeline for quantitatively measuring the intracellular drug distribution and its longitudinal uptake using a multimodal optical imaging platform, which can provide novel insights into the uptake pathways and target-sites.
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Jindou Shi, Prabuddha Mukherjee, Aneesh Alex, Edita Aksamitiene, Darold Spillman Jr., Marina Marjanovic, Minh Doan, Steve R. Hood, Stephen A. Boppart, "Single-cell analysis pipeline for quantification of intracellular drug distribution by CARS/2PF/FLIM," Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164911 (5 March 2021); https://doi.org/10.1117/12.2577041