Single molecule tracking and spectroscopy unveils molecular details in function and interactions of membrane receptors

Laura Marchetti; Fulvio Bonsignore; Rosy Amodeo; Chiara Schirripa Spagnolo; Aldo Moscardini; Francesco Gobbo; Antonino Cattaneo; Fabio Beltram; Stefano Luin

doi:10.1117/12.2578193

5 March 2021 Single molecule tracking and spectroscopy unveils molecular details in function and interactions of membrane receptors

Laura Marchetti, Fulvio Bonsignore, Rosy Amodeo, Chiara Schirripa Spagnolo, Aldo Moscardini, Francesco Gobbo, Antonino Cattaneo, Fabio Beltram, Stefano Luin

Author Affiliations +

Proceedings Volume 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV; 116500I (2021) https://doi.org/10.1117/12.2578193
Event: SPIE BiOS, 2021, Online Only

Abstract

We exploited, optimized, and developed various microscopy techniques for analyzing living matter at cellular and molecular levels. In particular, we developed a toolbox for single particle tracking (SPT) of membrane receptors and their ligands, suitable also for relatively fast single-pass membrane receptors; this is based on chemical tagging of recombinant proteins, TIRF microscopy, and automatized analysis of single particle trajectories. We are now extending it to simultaneous visualization and analysis of two moieties. The superresolved localization of this technique allowed analyzing functions, interactions and stoichiometry of (pro)neurotrophin receptors p75^NTR and TrkA and of their ligands in living cells, with particular attention on some of their existing or used mutants. The analysis also after treatments with ligands or drugs unraveled their mode of action in the first steps of sundry signaling pathways.

Conference Presentation

Citation Download Citation

Laura Marchetti, Fulvio Bonsignore, Rosy Amodeo, Chiara Schirripa Spagnolo, Aldo Moscardini, Francesco Gobbo, Antonino Cattaneo, Fabio Beltram, and Stefano Luin "Single molecule tracking and spectroscopy unveils molecular details in function and interactions of membrane receptors", Proc. SPIE 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV, 116500I (5 March 2021); https://doi.org/10.1117/12.2578193

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available