Presentation + Paper
12 April 2021 Correction of time-resolved SPAD array measurements for accurate single-photon time-resolved biological imaging
Author Affiliations +
Abstract
A 128x192 SPAD array (QuantiCam) with an on-chip time-to-digital converter in each pixel is used as a camera in a single-photon time-resolved fluorescence microscope. The SPAD array introduces systematic nonlinearities and timing offset to the measured photon arrival times. This limits the fidelity of the experimental results. A Monte-Carlo algorithm was developed to transform the raw photon time-stamp stream coming from the SPAD array into a corrected virtual “photon” time-stamp stream devoid of the systematic measurement errors. This data is compatible with existing downstream data processing pipelines used in time-correlated single-photon counting. We discuss the calibration measurement, the algorithm, their performance and application to live fluorescence lifetime imaging of photosynthetic organisms.
Conference Presentation
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jakub Nedbal, Francesco Mattioli Della Rocca, Richard Walker, Robert K. Henderson, and Klaus Suhling "Correction of time-resolved SPAD array measurements for accurate single-photon time-resolved biological imaging", Proc. SPIE 11721, Advanced Photon Counting Techniques XV, 117210T (12 April 2021); https://doi.org/10.1117/12.2587755
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KEYWORDS
Luminescence

Anisotropy

Biological research

Image sensors

Monte Carlo methods

Data acquisition

Microscopy

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