Silvia Romanohttps://orcid.org/0000-0002-8432-7611,1 Maria Mangini,2 Erika Penzo,3 Stefano Cabrini,3 Anna Chiara De Luca,2 Ivo Rendina,1 Vito Mocella,1 Gianluigi Zito1
1Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello" (Italy) 2Istituto di Biochimica e Biologia Cellulare (Italy) 3The Molecular Foundry (United States)
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A novel hyperspectral sensing imaging taking advantage of engineered all-dielectric metasurfaces supporting bound states in the continuum here is discussed. This approach combines surface-enhanced fluorescence and res- onant shift both based on high-Q resonances in proximity of bound states in the continuum. The amplification of the optical field on resonance allows increasing the fluorescence emission of a dye as a function of the spatial- variant dielectric environment in the near-field of the structure. We first demonstrate the fluorescence emission amplification by resonant pump matching in microscopy configuration. Then, we take advantage of Fano reso- nances in the fluorescence emission to map the spatially variant environment of biological cells. To demonstrate the real implementation of the proposed BIC-enhanced imaging as a platform for biosensing, hyperspectral maps of prostate cancer cells are experimentally reconstructed.
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Silvia Romano, Maria Mangini, Erika Penzo, Stefano Cabrini, Anna Chiara De Luca, Ivo Rendina, Vito Mocella, Gianluigi Zito, "Ultrasensitive surface refractive index imaging in all-dielectric structures," Proc. SPIE 11772, Optical Sensors 2021, 117720Y (18 April 2021); https://doi.org/10.1117/12.2589831